PM10对人肺成纤维细胞的影响
2009/07/09
哮喘的发病率呈不断增加的趋势，其中以新英格兰州最高。流行病学研究显示，大气颗粒物（PM）与哮喘发作的严重程度有关。但由于PM为一复杂的混合物质，因而分析具体的恶化因素较为困难。
本文主要研究NIST（美国国家标准与技术研究院）和缅因州的PM，以及PM中释放的可溶性金属对人肺成纤维细胞生长的影响。
NIST PM本身对细胞的生存率具有显著影响，来源于PM的可溶性金属同样影响细胞的存活。分别给予细胞10, 50, 100和 200 μg/cm2 PM处理，细胞存活率分别为84±13%，69 ±15%，58±14%和58±16%。我们检测了不同浓度的来源于NIST PM的8个酸溶性金属溶液对细胞存活率的影响，分别为91±11%，87±10%，72±18%和66±20%。提取缅因州PM可溶性金属物质，混合成不同的浓度，检测其对细胞存活率的影响，分别为88±5%，81±5%，79±3%和57±9%。这些金属物质中，我们检测了锰、铜、钒和砷对细胞的影响。在我们检测的浓度中，仅砷和钒对细胞存活率有影响。
（林江涛 审校）
Toxicol Ind Health. 2009 Mar;25(2):111-120
The effect of PM10 on human lung fibroblasts.
Alley D, Langley-Turnbaugh S, Gordon N, Wise J, Van Epps G, Jalbert A.
Department of Chemistry, University of Southern Maine, Gorham, Maine.
Asthma diagnoses are increasing nationally with the highest rates in the New England states. Epidemiological studies have suggested a relationship between airborne particulate matter (PM) and severity of an asthma attack. However, because particulate matter, PM, is such a complex mixture, it is difficult to isolate the exacerbating factors. In this paper we investigate the effect of NIST (National Institute of Standards and Technology) and Maine PM and the soluble metals released from the PM on the growth of human lung fibroblasts. While the NIST PM itself had the most pronounced effect on cell survival rates, solutions of metals extracted from the PM also affected cell survival. Treatment of cells with 10, 50, 100 and 200 ug/cm(2) resulted in 84 +/- 13%, 69 +/- 15%, 58 +/- 14% and 58 +/- 16% survival, respectively. Appropriate concentrations of eight acid soluble metals from NIST PM were determined and tested on cells giving 91 +/- 11%, 87 +/- 10%, 72 +/- 18% and 66 +/- 20% survival, respectively. Soluble metals from Maine PM were extracted and mixtures of appropriate concentrations of these metals were used to treat cells, resulting in 88 +/- 5%, 81 +/- 5%, 79 +/- 3% and 57 +/- 9% survival rate. To determine which, if any, of the metals individually affected the cells, Mn, Cu, V and As were used to treat the cells. At the metal concentrations tested, only As and V affected cell survival.
Asthma diagnoses are increasing nationally with the highest rates in the New England states. Epidemiological studies have suggested a relationship between airborne particulate matter (PM) and severity of an asthma attack. However, because particulate matter, PM, is such a complex mixture, it is difficult to isolate the exacerbating factors. In this paper we investigate the effect of NIST (National Institute of Standards and Technology) and Maine PM and the soluble metals released from the PM on the growth of human lung fibroblasts. While the NIST PM itself had the most pronounced effect on cell survival rates, solutions of metals extracted from the PM also affected cell survival. Treatment of cells with 10, 50, 100 and 200 ug/cm(2) resulted in 84 +/- 13%, 69 +/- 15%, 58 +/- 14% and 58 +/- 16% survival, respectively. Appropriate concentrations of eight acid soluble metals from NIST PM were determined and tested on cells giving 91 +/- 11%, 87 +/- 10%, 72 +/- 18% and 66 +/- 20% survival, respectively. Soluble metals from Maine PM were extracted and mixtures of appropriate concentrations of these metals were used to treat cells, resulting in 88 +/- 5%, 81 +/- 5%, 79 +/- 3% and 57 +/- 9% survival rate. To determine which, if any, of the metals individually affected the cells, Mn, Cu, V and As were used to treat the cells. At the metal concentrations tested, only As and V affected cell survival.
上一篇：
瘦素是否对肥胖-哮喘相关性起作用
下一篇：
过敏性支气管哮喘小鼠气道中的ADAM8上调
