与哮喘有关的炎症和重塑有关的生物标志物研究
2009/05/22
哮喘为一类复杂的呼吸道炎症性疾病。炎症细胞、肽类介质、细胞外基质以及蛋白酶等之间的相互作用参与了哮喘相关的炎症和重塑过程。到目前为止，尚未发现在哮喘的病理生理过程中起到重要作用的新的蛋白介质。
本研究对过敏原诱导的呼吸道炎症和重构模型小鼠的肺脏，采用表面增强激光解吸/电离飞行时间质谱（SELDI-TOF-MS）法进行检测，以期获得潜在的靶向蛋白。与对照组小鼠相比，模型小鼠中某些特异性表达的蛋白或肽出现显著变化。从中找到5个不同的蛋白：发现炎症区1或RELMalpha（FIZZ-1）、钙周期蛋白（S100A6）、clara细胞分泌蛋白（CC10）、泛素和组蛋白H4。
（林江涛审校）
Calvo FQ, et al. Proteomics. 2009 Mar 25. [Epub ahead of print]
Biomarker discovery in asthma-related inflammation and remodeling.
Asthma is a complex inflammatory disease of airways. A network of reciprocal interactions between inflammatory cells, peptidic mediators, extracellular matrix components, and proteases is thought to be involved in the installation and maintenance of asthma-related airway inflammation and remodeling. To date, new proteic mediators displaying significant activity in the pathophysiology of asthma are still to be unveiled. The main objective of this study was to uncover potential target proteins by using surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) on lung samples from mouse models of allergen-induced airway inflammation and remodeling. In this model, we pointed out several protein or peptide peaks that were preferentially expressed in diseased mice as compared to controls. We report the identification of different five proteins: found inflammatory zone 1 or RELMalpha (FIZZ-1), calcyclin (S100A6), clara cell secretory protein 10 (CC10), Ubiquitin, and Histone H4.
