哮喘和鼻炎患者诱导痰中子宫球蛋白相关蛋白1和Clara细胞蛋白的测定
2007/03/09
子宫球蛋白Uteroglobin(UG)是一种由类固醇类激素诱导分泌，进化上高度保守，具有免疫调节及抗炎等多种生物学功能的蛋白，它在组织中分布十分广泛，在外周血和尿中均可被检出，人的UG基因定位在染色体11q12.3～13。目前越来越多地发现，子宫球蛋白相关蛋白1Uteroglobin-related protein 1(UGRP1)和Clara细胞蛋白（Clara cell protein,CC16）在气道炎症反应过程中起着重要作用。
Claire de Burbure等哮喘和鼻炎各32例患者（变应性哮喘24例，变应性鼻炎20例）诱导痰标本的细胞学、UGRP1、CC16和白蛋白水平进行了测定，并选取了19例无过敏史的非吸烟者作为对照。结果表明，哮喘和鼻炎患者诱导痰中UGRP1增高，哮喘患者更为明显，尤其是变应性哮喘患者。而CC16虽与UGRP1呈正相关，但在上述两组患者中并无明显变化。诱导痰中UGRP1水平变化与渗透性变化（白蛋白水平增高）无关，与痰中巨噬细胞呈正相关，与嗜酸细胞呈负相关。痰中UGRP1和CC16的不同变化与负责其分泌的细胞类型不同有关，UGRP1主要在大的传导气道中分泌，CC16则主要由鼻腔和周围气道上皮分泌。
作者认为，哮喘和鼻炎患者诱导痰中UGRP1增高，提示其中气道炎症过程中可能起一定作用。
Claire de Burbure等哮喘和鼻炎各32例患者（变应性哮喘24例，变应性鼻炎20例）诱导痰标本的细胞学、UGRP1、CC16和白蛋白水平进行了测定，并选取了19例无过敏史的非吸烟者作为对照。结果表明，哮喘和鼻炎患者诱导痰中UGRP1增高，哮喘患者更为明显，尤其是变应性哮喘患者。而CC16虽与UGRP1呈正相关，但在上述两组患者中并无明显变化。诱导痰中UGRP1水平变化与渗透性变化（白蛋白水平增高）无关，与痰中巨噬细胞呈正相关，与嗜酸细胞呈负相关。痰中UGRP1和CC16的不同变化与负责其分泌的细胞类型不同有关，UGRP1主要在大的传导气道中分泌，CC16则主要由鼻腔和周围气道上皮分泌。
作者认为，哮喘和鼻炎患者诱导痰中UGRP1增高，提示其中气道炎症过程中可能起一定作用。
（赵海涛 沈阳军区总医院呼吸内科 110016 摘译）
(CHEST, 2007，131:172-179)
Uteroglobin-Related Protein 1 and Clara Cell Protein
in Induced Sputum of Patients With Asthma and Rhinitis*
Claire de Burbure, MD; Patrizia Pignatti, MD; Massimo Corradi, MD; Mario Malerba, MD; André Clippe, BSc; Xavier Dumont, BSc; Gianna Moscato, MD; Antonio Mutti, MD and Alfred Bernard, PhD?
Abstract
Rationale: Uteroglobin-related protein 1 (UGRP1) and Clara cell protein (CC16), members of the secretoglobin family, increasingly appear to play a role in airway inflammatory response.
Objective: To explore levels of UGRP1 and CC16 in induced sputum of patients with asthma and rhinitis.
Methods: Induced-sputum samples of patients with asthma or rhinitis (n = 32 each; atopic asthma, n = 24; atopic rhinitis, n = 20) and from 19 nonsmoking nonatopic control subjects were analyzed for cytology and levels of UGRP1, CC16, and albumin.
Measurements and main results: Sputum UGRP1 increased in both asthma and rhinitis, most strikingly so in asthma, in which changes were most significant in atopic individuals. By contrast, sputum CC16 did not change significantly in either condition, although it was positively correlated with UGRP1 in patients and control subjects. Changes in sputum UGRP1 in atopic asthma were not linked to permeability changes reflected by increased albumin levels but correlated positively with sputum macrophages and negatively with eosinophils. The observed differences in UGRP1 and CC16 may be linked to different cell populations being responsible for their secretion; UGRP1 is mainly secreted in larger conducting airways, whereas CC16 is mainly secreted by the nasal and peripheral airways epithelium.
Conclusions: The increase in UGRP1 but not of CC16 in asthma and rhinitis suggests that UGRP1 may play a role in these inflammatory diseases.
Key Words: asthma · Clara cell protein ·induced sputum ·rhinitis· uteroglobin-related protein 1
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血管生成素Ⅰ和血管生成素Ⅱ对哮喘患者气道血管通透性的作用
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