高盐饮食对人类和小鼠哮喘的影响：对特定T细胞特征和微生物组的作用
2025/01/06
摘要
背景：哮喘发病率的上升与不同的环境和生活方式有关，其中包括饮食习惯。然而，饮食中的盐分是否会导致哮喘发病仍有争议。本研究旨在探讨高盐摄入对人类哮喘发病率的影响，并使用小鼠模型评估其潜在机制。
方法：流行病学研究通过英国生物银行资源进行。数据来自42,976名有过敏史的参与者。通过随机尿液估算24小时钠排泄量，并通过Cox回归分析（校正相关协变量）评估其与哮喘发病率的相关性。在机制研究中，使用尘螨诱导的过敏性气道炎症（AAI）小鼠模型，分别采用高盐饮食（HSD）或正常盐食物喂养，评估疾病的发展情况。通过基于16S rRNA基因的宏条形码方法分析肺和粪便（作为肠道的替代物）的微生物组。
结果：在人类中，尿钠排泄与女性哮喘发病率直接相关，而与男性无关。HSD喂养的雌性小鼠表现出加重的AAI，其特征是总IgE水平升高，TH2-TH17偏向的炎症细胞浸润，并伴随着渗透敏感应激基因的上调。HSD诱导血清短链脂肪酸以及肠道和肺部微生物组发生明显变化，表现为肠道中拟杆菌门与厚壁菌门比例降低，乳酸杆菌相对丰度减少，而肺部富集的γ-变形菌门成员增加。
结论：在有过敏史的成年女性中，高食盐摄入量与哮喘发病率相关。雌性小鼠中示HSD诱导了肺部T细胞特征以及肠道和肺部微生物组的改变。
The impact of high-salt diet on asthma in humans and mice: Effect on specific T-cell signatures and microbiome
Allergy. 2024 May 26.
Musiol S, Harris CP, Gschwendtner S, Burrell A, Amar Y, Schnautz B, Renisch D, Braun SC, Haak S, Schloter M, Schmidt-Weber CB, Zielinski CE, Alessandrini F. The impact of high-salt diet on asthma in humans and mice: Effect on specific T-cell signatures and microbiome.
Abstract
Background: The rise in asthma has been linked to different environmental and lifestyle factors including dietary habits. Whether dietary salt contributes to asthma incidence, remains controversial. We aimed to investigate the impact of higher salt intake on asthma incidence in humans and to evaluate underlying mechanisms using mouse models.
Methods: Epidemiological research was conducted using the UK Biobank Resource. Data were obtained from 42,976 participants with a history of allergies. 24-h sodium excretion was estimated from spot urine, and its association with asthma incidence was assessed by Cox regression, adjusting for relevant covariates. For mechanistic studies, a mouse model of mite-induced allergic airway inflammation (AAI) fed with high-salt diet (HSD) or normal-salt chow was used to characterize disease development. The microbiome of lung and feces (as proxy for gut) was analyzed via 16S rRNA gene based metabarcoding approach.
Results: In humans, urinary sodium excretion was directly associated with asthma incidence among females but not among males. HSD-fed female mice displayed an aggravated AAI characterized by increased levels of total IgE, a TH2-TH17-biased inflammatory cell infiltration accompanied by upregulation of osmosensitive stress genes. HSD induced distinct changes in serum short chain fatty acids and in both gut and lung microbiome, with a lower Bacteroidetes to Firmicutes ratio and decreased Lactobacillus relative abundance in the gut, and enriched members of Gammaproteobacteria in the lung.
Conclusions: High dietary salt consumption correlates with asthma incidence in female adults with a history of allergies. Female mice revealed HSD-induced T-cell lung profiles accompanied by alterations of gut and lung microbiome.
背景：哮喘发病率的上升与不同的环境和生活方式有关，其中包括饮食习惯。然而，饮食中的盐分是否会导致哮喘发病仍有争议。本研究旨在探讨高盐摄入对人类哮喘发病率的影响，并使用小鼠模型评估其潜在机制。
方法：流行病学研究通过英国生物银行资源进行。数据来自42,976名有过敏史的参与者。通过随机尿液估算24小时钠排泄量，并通过Cox回归分析（校正相关协变量）评估其与哮喘发病率的相关性。在机制研究中，使用尘螨诱导的过敏性气道炎症（AAI）小鼠模型，分别采用高盐饮食（HSD）或正常盐食物喂养，评估疾病的发展情况。通过基于16S rRNA基因的宏条形码方法分析肺和粪便（作为肠道的替代物）的微生物组。
结果：在人类中，尿钠排泄与女性哮喘发病率直接相关，而与男性无关。HSD喂养的雌性小鼠表现出加重的AAI，其特征是总IgE水平升高，TH2-TH17偏向的炎症细胞浸润，并伴随着渗透敏感应激基因的上调。HSD诱导血清短链脂肪酸以及肠道和肺部微生物组发生明显变化，表现为肠道中拟杆菌门与厚壁菌门比例降低，乳酸杆菌相对丰度减少，而肺部富集的γ-变形菌门成员增加。
结论：在有过敏史的成年女性中，高食盐摄入量与哮喘发病率相关。雌性小鼠中示HSD诱导了肺部T细胞特征以及肠道和肺部微生物组的改变。
(四川大学华西医院呼吸与危重症医学科 吴雯雯1 王霁1 王刚1 译)
(Allergy. 2024 May 26.)
(Allergy. 2024 May 26.)
The impact of high-salt diet on asthma in humans and mice: Effect on specific T-cell signatures and microbiome
Allergy. 2024 May 26.
Musiol S, Harris CP, Gschwendtner S, Burrell A, Amar Y, Schnautz B, Renisch D, Braun SC, Haak S, Schloter M, Schmidt-Weber CB, Zielinski CE, Alessandrini F. The impact of high-salt diet on asthma in humans and mice: Effect on specific T-cell signatures and microbiome.
Abstract
Background: The rise in asthma has been linked to different environmental and lifestyle factors including dietary habits. Whether dietary salt contributes to asthma incidence, remains controversial. We aimed to investigate the impact of higher salt intake on asthma incidence in humans and to evaluate underlying mechanisms using mouse models.
Methods: Epidemiological research was conducted using the UK Biobank Resource. Data were obtained from 42,976 participants with a history of allergies. 24-h sodium excretion was estimated from spot urine, and its association with asthma incidence was assessed by Cox regression, adjusting for relevant covariates. For mechanistic studies, a mouse model of mite-induced allergic airway inflammation (AAI) fed with high-salt diet (HSD) or normal-salt chow was used to characterize disease development. The microbiome of lung and feces (as proxy for gut) was analyzed via 16S rRNA gene based metabarcoding approach.
Results: In humans, urinary sodium excretion was directly associated with asthma incidence among females but not among males. HSD-fed female mice displayed an aggravated AAI characterized by increased levels of total IgE, a TH2-TH17-biased inflammatory cell infiltration accompanied by upregulation of osmosensitive stress genes. HSD induced distinct changes in serum short chain fatty acids and in both gut and lung microbiome, with a lower Bacteroidetes to Firmicutes ratio and decreased Lactobacillus relative abundance in the gut, and enriched members of Gammaproteobacteria in the lung.
Conclusions: High dietary salt consumption correlates with asthma incidence in female adults with a history of allergies. Female mice revealed HSD-induced T-cell lung profiles accompanied by alterations of gut and lung microbiome.
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妊娠期间颗粒物暴露通过胎盘表观遗传变化影响儿童哮喘：神经元分化和增殖和Notch信号通路
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