β-葡聚糖纳米颗粒可通过抑制小鼠铁死亡及DNA损伤缓解哮喘急性发作
2024/09/29
摘要
背景：哮喘是一种严重的呼吸道疾病，其特征是气道炎症、重塑和氧化应激。β-葡聚糖（BG）是真菌细胞结构的多糖成分，具有强大的免疫调节活性。
目的：本研究旨在探究β-葡聚糖纳米颗粒（BG-NP）对卵清蛋白（OVA）诱导过敏性哮喘小鼠模型的抗哮喘及抗铁死亡的作用。
方法：BG是桦褐孔菌（Inonotus obliquus）的提取物。BG-NP技术特征包括FT-IR、UV-可见光谱、ζ电位分析、DLS、XRD和TEM等。本研究将Balb/C小鼠分为以下五组：对照组、未治疗哮喘组、地塞米松（Dexa）治疗组（1mg/kg）、BG治疗组（100mg/kg）和BG-NPs治疗组（45mg/kg）。
结果：BG-NP可通过降低丙二醛（MDA）水平，提高还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平，显著减少呼吸道炎症细胞浸润，同时降低血清IgE浓度、DNA损伤程度和氧化应激标志物水平。此外，BG -NP可减少铁沉积，增强肺组织细胞中GPx4基因的转录活性，减轻铁死亡。
结论：结果表明，BG-NP可通过抑制氧化应激、炎症、DNA损伤和铁死亡来减轻哮喘，BG NP可作为过敏性哮喘的潜在治疗方法。
β-glucan nanoparticles alleviate acute asthma by suppressing ferroptosis and DNA damage in mice.
Ebeed BW, Abdelmawgood IA, Kotb MA, Mahana NA, Mohamed AS, Ramadan MA, Badr AM, Nasr M, Qurani OM, Hamdy RM, El-Hakiem NYA, Fahim MK, Fekry MM, Eid JI.
Abstract
BACKGROUND:Asthma is a severe respiratory disease marked by airway inflammation, remodeling, and oxidative stress. β-Glucan (BG), a polysaccharide constituent of fungal cellular structures, exhibits potent immunomodulatory activities.
OBJECTIVE:The investigational focus was on the anti-asthmatic and anti-ferroptotic properties of beta-glucan nanoparticles (BG-NPs) in a murine model of allergic asthma induced by ovalbumin (OVA).
METHODS:BG was extracted from Chaga mushrooms (Inonotus obliquus), and its BG-NPs were characterized utilizing techniques including FT-IR, UV visible spectroscopy, zeta potential analysis, DLS, XRD, and TEM. The Balb/C mice were allocated into five groups: control, untreated asthmatic, dexamethasone (Dexa)-treated (1 mg/kg), BG-treated (100 mg/kg), and BG-NPs-treated (45 mg/kg).
RESULTS:Treatment with BG-NPs markedly diminished the entry of inflammatory cells into the respiratory passage, serum IgE concentrations, DNA damage, and markers of oxidative stress through the reduction of malonaldehyde (MDA) levels and enhancing the levels of reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). Furthermore, BG-NPs reduced iron deposition and promoted the transcriptional activity of the GPx4 gene in pulmonary cells, attenuating ferroptosis.
CONCLUSION:The results demonstrated that BG-NPs reduced asthma by inhibiting oxidative stress, inflammation, DNA damage, and ferroptosis. Our results suggest that BG-NPs could be used as potential treatments for allergic asthma.
背景：哮喘是一种严重的呼吸道疾病，其特征是气道炎症、重塑和氧化应激。β-葡聚糖（BG）是真菌细胞结构的多糖成分，具有强大的免疫调节活性。
目的：本研究旨在探究β-葡聚糖纳米颗粒（BG-NP）对卵清蛋白（OVA）诱导过敏性哮喘小鼠模型的抗哮喘及抗铁死亡的作用。
方法：BG是桦褐孔菌（Inonotus obliquus）的提取物。BG-NP技术特征包括FT-IR、UV-可见光谱、ζ电位分析、DLS、XRD和TEM等。本研究将Balb/C小鼠分为以下五组：对照组、未治疗哮喘组、地塞米松（Dexa）治疗组（1mg/kg）、BG治疗组（100mg/kg）和BG-NPs治疗组（45mg/kg）。
结果：BG-NP可通过降低丙二醛（MDA）水平，提高还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平，显著减少呼吸道炎症细胞浸润，同时降低血清IgE浓度、DNA损伤程度和氧化应激标志物水平。此外，BG -NP可减少铁沉积，增强肺组织细胞中GPx4基因的转录活性，减轻铁死亡。
结论：结果表明，BG-NP可通过抑制氧化应激、炎症、DNA损伤和铁死亡来减轻哮喘，BG NP可作为过敏性哮喘的潜在治疗方法。
(中日友好医院呼吸与危重症医学科 张婧媛 摘译 林江涛 审校)
(Apoptosis. 2024 Sep 21. doi: 10.1007/s10495-024-02013-9. Epub ahead of print. PMID: 39305381.)
(Apoptosis. 2024 Sep 21. doi: 10.1007/s10495-024-02013-9. Epub ahead of print. PMID: 39305381.)
β-glucan nanoparticles alleviate acute asthma by suppressing ferroptosis and DNA damage in mice.
Ebeed BW, Abdelmawgood IA, Kotb MA, Mahana NA, Mohamed AS, Ramadan MA, Badr AM, Nasr M, Qurani OM, Hamdy RM, El-Hakiem NYA, Fahim MK, Fekry MM, Eid JI.
Abstract
BACKGROUND:Asthma is a severe respiratory disease marked by airway inflammation, remodeling, and oxidative stress. β-Glucan (BG), a polysaccharide constituent of fungal cellular structures, exhibits potent immunomodulatory activities.
OBJECTIVE:The investigational focus was on the anti-asthmatic and anti-ferroptotic properties of beta-glucan nanoparticles (BG-NPs) in a murine model of allergic asthma induced by ovalbumin (OVA).
METHODS:BG was extracted from Chaga mushrooms (Inonotus obliquus), and its BG-NPs were characterized utilizing techniques including FT-IR, UV visible spectroscopy, zeta potential analysis, DLS, XRD, and TEM. The Balb/C mice were allocated into five groups: control, untreated asthmatic, dexamethasone (Dexa)-treated (1 mg/kg), BG-treated (100 mg/kg), and BG-NPs-treated (45 mg/kg).
RESULTS:Treatment with BG-NPs markedly diminished the entry of inflammatory cells into the respiratory passage, serum IgE concentrations, DNA damage, and markers of oxidative stress through the reduction of malonaldehyde (MDA) levels and enhancing the levels of reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). Furthermore, BG-NPs reduced iron deposition and promoted the transcriptional activity of the GPx4 gene in pulmonary cells, attenuating ferroptosis.
CONCLUSION:The results demonstrated that BG-NPs reduced asthma by inhibiting oxidative stress, inflammation, DNA damage, and ferroptosis. Our results suggest that BG-NPs could be used as potential treatments for allergic asthma.
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高CBD提取物（CBD-X）在哮喘管理中的作用：降低Th2驱动的细胞因子分泌和中性粒细胞/嗜酸性粒细胞活性
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探索淋巴细胞的CD26-/lo亚群在哮喘表型和严重程度中的作用：一种表达原型粒细胞蛋白的新型CD4+T细胞亚群
