伴有症状性嗜酸性粒细胞炎症的成人哮喘肠道微生物组的改变
2023/03/21
摘要
背景：越来越多的证据表明，肠道微生物组与哮喘有关。然而，成人哮喘中肠道微生物组的改变尚未得到充分证实。我们旨在研究有症状嗜酸性粒细胞炎症的成人哮喘患者的肠道微生物组特征。
方法：将症状性嗜酸性粒细胞性哮喘组（EA，N=28）粪便的16s rRNA基因宏基因组分析与健康对照组（HC，N=18）和慢性咳嗽对照组（CC，N=13）进行比较。在EA组中进行了个体分类群和临床标志物之间的相关性分析。在EA组症状显著改善的患者中，检查了肠道微生物组的变化。
结果：电针组的钩端螺旋菌科和示波螺旋菌科的相对丰度显著降低，拟杆菌类增加。在电针组中，钩端螺旋体科与2型炎症和肺功能下降的指标呈负相关。肠杆菌科和普雷沃氏菌分别与2型炎症和肺功能下降呈正相关。EA组与氨基酸代谢和次级胆汁酸生物合成相关的预测基因的丰度降低。这些功能性基因家族的改变可能与肠道通透性有关，而电针组的血清脂多糖浓度实际上很高。1个月后症状改善的EA患者肠道微生物组没有明显变化。
结论：有症状的嗜酸性粒细胞性成人哮喘患者肠道微生物组组成发生改变。具体而言，观察到共生梭状芽胞杆菌的减少，且拉科螺旋杆菌的减少与血液嗜酸性粒细胞增多和肺功能下降相关。
Adult asthma with symptomatic eosinophilic inflammation is accompanied by alteration in gut microbiome
Bon-Hee Gu, Jun-Pyo Choi, Tansol Park, A-Sol Kim, Ho Young Jung, Doo Young Choi, Sang Jin Lee, Yoon-Seok Chang, Myunghoo Kim, Han-Ki Park
Abstract
Background: Accumulating evidence suggests that the gut microbiome is associated with asthma. However, altered gut microbiome in adult asthma is not yet well established. We aimed to investigate the gut microbiome profiles of adult asthmatic patients with symptomatic eosinophilic inflammation.
Methods: The 16s rRNA gene metagenomic analysis of feces in the symptomatic eosinophilic asthma group (EA, N=28) was compared with the healthy control (HC, N=18) and the chronic cough control (CC, N=13). A correlation analysis between individual taxa and clinical markers was performed within the EA group. Changes in the gut microbiome were examined in patients with significant symptom improvement in the EA group.
Results: The relative abundances of Lachnospiraceae and Oscillospiraceae significantly decreased and Bacteroidetes increased in the EA group. Within EA group, Lachnospiraceae was negatively correlated with indicators of type 2 inflammation and lung function decline. Enterobacteriaceae and Prevotella was positively associated with type 2 inflammation and lung function decline, respectively. The abundance of predicted genes associated with amino acid metabolism and secondary bile acid biosynthesis was diminished in the EA group. These functional gene family alterations could be related to gut permeability, and the serum lipopolysaccharide concentration was actually high in the EA group. EA patients with symptom improvement after 1 month did not show a significant change in the gut microbiome.
Conclusions: Symptomatic eosinophilic adult asthma patients showed altered the gut microbiome composition. Specifically, a decrease in commensal clostridia was observed, and a decrease in Lachnospiraceae was correlated with blood eosinophilia and lung function decline
背景：越来越多的证据表明，肠道微生物组与哮喘有关。然而，成人哮喘中肠道微生物组的改变尚未得到充分证实。我们旨在研究有症状嗜酸性粒细胞炎症的成人哮喘患者的肠道微生物组特征。
方法：将症状性嗜酸性粒细胞性哮喘组（EA，N=28）粪便的16s rRNA基因宏基因组分析与健康对照组（HC，N=18）和慢性咳嗽对照组（CC，N=13）进行比较。在EA组中进行了个体分类群和临床标志物之间的相关性分析。在EA组症状显著改善的患者中，检查了肠道微生物组的变化。
结果：电针组的钩端螺旋菌科和示波螺旋菌科的相对丰度显著降低，拟杆菌类增加。在电针组中，钩端螺旋体科与2型炎症和肺功能下降的指标呈负相关。肠杆菌科和普雷沃氏菌分别与2型炎症和肺功能下降呈正相关。EA组与氨基酸代谢和次级胆汁酸生物合成相关的预测基因的丰度降低。这些功能性基因家族的改变可能与肠道通透性有关，而电针组的血清脂多糖浓度实际上很高。1个月后症状改善的EA患者肠道微生物组没有明显变化。
结论：有症状的嗜酸性粒细胞性成人哮喘患者肠道微生物组组成发生改变。具体而言，观察到共生梭状芽胞杆菌的减少，且拉科螺旋杆菌的减少与血液嗜酸性粒细胞增多和肺功能下降相关。
(中日友好医院呼吸与危重症医学科 李红雯 摘译 林江涛 审校)
(Allergy. 2023 Feb 27. doi: 10.1111/all.15691.)
(Allergy. 2023 Feb 27. doi: 10.1111/all.15691.)
Adult asthma with symptomatic eosinophilic inflammation is accompanied by alteration in gut microbiome
Bon-Hee Gu, Jun-Pyo Choi, Tansol Park, A-Sol Kim, Ho Young Jung, Doo Young Choi, Sang Jin Lee, Yoon-Seok Chang, Myunghoo Kim, Han-Ki Park
Abstract
Background: Accumulating evidence suggests that the gut microbiome is associated with asthma. However, altered gut microbiome in adult asthma is not yet well established. We aimed to investigate the gut microbiome profiles of adult asthmatic patients with symptomatic eosinophilic inflammation.
Methods: The 16s rRNA gene metagenomic analysis of feces in the symptomatic eosinophilic asthma group (EA, N=28) was compared with the healthy control (HC, N=18) and the chronic cough control (CC, N=13). A correlation analysis between individual taxa and clinical markers was performed within the EA group. Changes in the gut microbiome were examined in patients with significant symptom improvement in the EA group.
Results: The relative abundances of Lachnospiraceae and Oscillospiraceae significantly decreased and Bacteroidetes increased in the EA group. Within EA group, Lachnospiraceae was negatively correlated with indicators of type 2 inflammation and lung function decline. Enterobacteriaceae and Prevotella was positively associated with type 2 inflammation and lung function decline, respectively. The abundance of predicted genes associated with amino acid metabolism and secondary bile acid biosynthesis was diminished in the EA group. These functional gene family alterations could be related to gut permeability, and the serum lipopolysaccharide concentration was actually high in the EA group. EA patients with symptom improvement after 1 month did not show a significant change in the gut microbiome.
Conclusions: Symptomatic eosinophilic adult asthma patients showed altered the gut microbiome composition. Specifically, a decrease in commensal clostridia was observed, and a decrease in Lachnospiraceae was correlated with blood eosinophilia and lung function decline
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