NCR(+)ILC3s 与哮喘严重程度相关的气道微生物组之间的相互作用
2021/10/20
摘要
背景：哮喘是一种异质性疾病，其发展受到多种环境和遗传因素的影响。虽然最近的几项研究表明肠道微生物失调可能促进哮喘，但对最近发现的肺微生物群和哮喘之间的关系知之甚少。先天淋巴样细胞(ILCs)最近也被证明参与哮喘。
方法：为了探讨肺微生物组、ILCs与哮喘的关系，我们招募了23例健康对照(HC)、42例非重度哮喘患者和32例重度哮喘患者。
结果：流式细胞术分析显示，严重哮喘与肺中较少的自然细胞毒性受体(NCR)(+)ILC3s相关。在其他ILC亚群、巨噬细胞和单核细胞中未观察到类似的变化。哮喘患者的肺和肠道微生物群落的α和β多样性与HC没有区别。然而，肺功能与NCR(+)ILC3频率和肺内微生物多样性呈正相关。在HC中，痰NCR(+)ILC3频率与肺微生物组多样性呈正相关，而在重度哮喘中则相反。
结论：总之，这些数据表明，气道NCR(+)ILC3s可能有助于健康的共生多样性和正常的肺功能。
Interactions between NCR(+)ILC3s and the Microbiome in the Airways Shape Asthma Severity
Ham J, Kim J, Choi S, et al
Abstract
BACKGROUND:Asthma is a heterogeneous disease whose development is shaped by a variety of environmental and genetic factors. While several recent studies suggest that microbial dysbiosis in the gut may promote asthma, little is known about the relationship between the recently discovered lung microbiome and asthma.Innate lymphoid cells (ILCs) have also been shown recently to participate in asthma.
METHODS:To investigate the relationship between the lung microbiome, ILCs, and asthma, we recruited 23 healthy controls(HC), 42 patients with non-severe asthma, and 32 patients with severe asthma.
RESULTS:Flow cytometry analysis showed severe asthma associated with fewer natural cytotoxicity receptor (NCR)(+)ILC3s in the lung. Similar changes in other ILC subsets, macrophages, and monocytes were not observed. The asthma patients did not differ from the HC in terms of the alpha and beta-diversity of the lung and gut microbiomes. However, lung function correlated positively with both NCR(+)ILC3 frequencies and microbial diversity in the lung. Sputum NCR(+)ILC3 frequencies correlated positively with lung microbiome diversity in the HC, but this relationship was inversed in severe asthma.
CONCLUSIONS:Together, these data suggest that airway NCR(+)ILC3s may contribute to a healthy commensal diversity and normal lung function.
背景：哮喘是一种异质性疾病，其发展受到多种环境和遗传因素的影响。虽然最近的几项研究表明肠道微生物失调可能促进哮喘，但对最近发现的肺微生物群和哮喘之间的关系知之甚少。先天淋巴样细胞(ILCs)最近也被证明参与哮喘。
方法：为了探讨肺微生物组、ILCs与哮喘的关系，我们招募了23例健康对照(HC)、42例非重度哮喘患者和32例重度哮喘患者。
结果：流式细胞术分析显示，严重哮喘与肺中较少的自然细胞毒性受体(NCR)(+)ILC3s相关。在其他ILC亚群、巨噬细胞和单核细胞中未观察到类似的变化。哮喘患者的肺和肠道微生物群落的α和β多样性与HC没有区别。然而，肺功能与NCR(+)ILC3频率和肺内微生物多样性呈正相关。在HC中，痰NCR(+)ILC3频率与肺微生物组多样性呈正相关，而在重度哮喘中则相反。
结论：总之，这些数据表明，气道NCR(+)ILC3s可能有助于健康的共生多样性和正常的肺功能。
（中日友好医院呼吸与危重症医学科 李春晓 摘译 林江涛 审校）
（Immune Netw, 2021, 21(4): e25）
（Immune Netw, 2021, 21(4): e25）
Interactions between NCR(+)ILC3s and the Microbiome in the Airways Shape Asthma Severity
Ham J, Kim J, Choi S, et al
Abstract
BACKGROUND:Asthma is a heterogeneous disease whose development is shaped by a variety of environmental and genetic factors. While several recent studies suggest that microbial dysbiosis in the gut may promote asthma, little is known about the relationship between the recently discovered lung microbiome and asthma.Innate lymphoid cells (ILCs) have also been shown recently to participate in asthma.
METHODS:To investigate the relationship between the lung microbiome, ILCs, and asthma, we recruited 23 healthy controls(HC), 42 patients with non-severe asthma, and 32 patients with severe asthma.
RESULTS:Flow cytometry analysis showed severe asthma associated with fewer natural cytotoxicity receptor (NCR)(+)ILC3s in the lung. Similar changes in other ILC subsets, macrophages, and monocytes were not observed. The asthma patients did not differ from the HC in terms of the alpha and beta-diversity of the lung and gut microbiomes. However, lung function correlated positively with both NCR(+)ILC3 frequencies and microbial diversity in the lung. Sputum NCR(+)ILC3 frequencies correlated positively with lung microbiome diversity in the HC, but this relationship was inversed in severe asthma.
CONCLUSIONS:Together, these data suggest that airway NCR(+)ILC3s may contribute to a healthy commensal diversity and normal lung function.
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