塑料微粒暴露对正常和哮喘肺生理的有害影响
2021/09/24
摘要
背景：人们越来越担心空气中的微塑料(MP)可能对人类健康有害。然而，关于MP对呼吸系统影响的研究还很有限。
方法：我们测试了MP暴露对小鼠正常和哮喘肺生理的影响。
结果：我们发现MP暴露可导致正常小鼠肺炎症细胞浸润、支气管肺泡巨噬细胞聚集、支气管肺泡灌洗液(BALF)中TNF-α水平升高、血浆IgG1生成增加。MP暴露也通过增加粘液分泌和炎症细胞浸润(巨噬细胞明显聚集)而影响哮喘症状。进一步，我们发现巨噬细胞标记物与MP结合荧光，表明MP被巨噬细胞吞噬。比较转录组分析显示，MP暴露改变了与免疫反应、细胞应激反应和程序性细胞死亡相关的基因簇。生物信息学分析进一步揭示了MP刺激肿瘤坏死因子和免疫球蛋白的产生以激活一组跨膜B细胞抗原的分子机制，从而导致哮喘模型中细胞应激和程序性细胞死亡的调节。
结论：综上所述，我们发现MP暴露对健康小鼠和哮喘小鼠的呼吸系统都有有害影响，这就迫切需要讨论和行动来减轻环境塑料微粒污染物。
Detrimental effects of microplastic exposure on normal and asthmatic pulmonary physiology
Lu K, Lai KP, Stoeger T, et al.
Abstract
Background:Concerns that airborne microplastics (MP) may be detrimental to human health are rising. However, research on the effects of MP on the respiratory system are limited.
Methods:We tested the effect of MP exposure on both normal and asthmatic pulmonary physiology in mice.
Results：We show that MP exposure caused pulmonary inflammatory cell infiltration, bronchoalveolar macrophage aggregation, increased TNF-α level in bronchoalveolar lavage fluid (BALF), and increased plasma IgG1 production in normal mice. MP exposure also affected asthma symptoms by increasing mucus production and inflammatory cell infiltration with notable macrophage aggregation. Further, we found co-labeling of macrophage markers with MP incorporating fluorescence, which indicates phagocytosis of the MP by macrophages. A comparative transcriptomic analysis showed that MP exposure altered clusters of genes related to immune response, cellular stress response, and programmed cell death. A bioinformatics analysis further uncovered the molecular mechanism whereby MP stimulated production of tumor necrosis factor and immunoglobulins to activate a group of transmembrane B-cell antigens, leading to the modulation of cellular stress and programmed cell death in the asthma model.
Conclusions:In summary, we show that MP exposure had detrimental effects on the respiratory system in both healthy and asthmatic mice, which calls for urgent discourse and action to mitigate environmental microplastic pollutants.
背景：人们越来越担心空气中的微塑料(MP)可能对人类健康有害。然而，关于MP对呼吸系统影响的研究还很有限。
方法：我们测试了MP暴露对小鼠正常和哮喘肺生理的影响。
结果：我们发现MP暴露可导致正常小鼠肺炎症细胞浸润、支气管肺泡巨噬细胞聚集、支气管肺泡灌洗液(BALF)中TNF-α水平升高、血浆IgG1生成增加。MP暴露也通过增加粘液分泌和炎症细胞浸润(巨噬细胞明显聚集)而影响哮喘症状。进一步，我们发现巨噬细胞标记物与MP结合荧光，表明MP被巨噬细胞吞噬。比较转录组分析显示，MP暴露改变了与免疫反应、细胞应激反应和程序性细胞死亡相关的基因簇。生物信息学分析进一步揭示了MP刺激肿瘤坏死因子和免疫球蛋白的产生以激活一组跨膜B细胞抗原的分子机制，从而导致哮喘模型中细胞应激和程序性细胞死亡的调节。
结论：综上所述，我们发现MP暴露对健康小鼠和哮喘小鼠的呼吸系统都有有害影响，这就迫切需要讨论和行动来减轻环境塑料微粒污染物。
（中日友好医院呼吸与危重症医学科 李春晓 摘译 林江涛 审校）
（Journal of Hazardous Materials, 2021, 416.）
（Journal of Hazardous Materials, 2021, 416.）
Detrimental effects of microplastic exposure on normal and asthmatic pulmonary physiology
Lu K, Lai KP, Stoeger T, et al.
Abstract
Background:Concerns that airborne microplastics (MP) may be detrimental to human health are rising. However, research on the effects of MP on the respiratory system are limited.
Methods:We tested the effect of MP exposure on both normal and asthmatic pulmonary physiology in mice.
Results：We show that MP exposure caused pulmonary inflammatory cell infiltration, bronchoalveolar macrophage aggregation, increased TNF-α level in bronchoalveolar lavage fluid (BALF), and increased plasma IgG1 production in normal mice. MP exposure also affected asthma symptoms by increasing mucus production and inflammatory cell infiltration with notable macrophage aggregation. Further, we found co-labeling of macrophage markers with MP incorporating fluorescence, which indicates phagocytosis of the MP by macrophages. A comparative transcriptomic analysis showed that MP exposure altered clusters of genes related to immune response, cellular stress response, and programmed cell death. A bioinformatics analysis further uncovered the molecular mechanism whereby MP stimulated production of tumor necrosis factor and immunoglobulins to activate a group of transmembrane B-cell antigens, leading to the modulation of cellular stress and programmed cell death in the asthma model.
Conclusions:In summary, we show that MP exposure had detrimental effects on the respiratory system in both healthy and asthmatic mice, which calls for urgent discourse and action to mitigate environmental microplastic pollutants.
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诱导痰中MMP12 mRNA表达增加与哮喘患者气道嗜酸性炎症相关:来自生物信息学分析和实验验证的证据
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