在哮喘急性发作期间，外周血中Th2细胞标志物增多
2021/05/19
摘要
背景：过敏性哮喘的特征是2型炎症。我们已经表明，重症哮喘患者与频繁急性发作的患者存在2型炎症的增加。然而，目前尚不清楚2型炎症的增加是否会导致哮喘急性发作。本研究旨在确定因哮喘急性发作到急诊科(ED)就诊患者的Th2免疫参数，并将这些参数与哮喘的临床和生理指标联系起来。
方法：在给予知情同意后，招募16例因哮喘急性发作到急诊科(ED)就诊的成人哮喘患者。10例受试者2周后返回进行随访。在两次随访期间收集生理参数、哮喘控制问卷(ACQ6)、哮喘生活质量问卷(AQLQ)和静脉血。通过全血流式细胞术对免疫细胞进行分析：CD4+ T细胞、Th2细胞(CD4+ CRTh2+ T细胞和表达CRTh2的CD4+ T细胞%)、嗜酸性粒细胞和2型固有淋巴细胞(ILC2)。
结果：在急性发作期间，外周血Th2细胞数量与ACQ6和AQLQ评分相关，而与ILC2和嗜酸性粒细胞数量不相关。与随访期间相比，急性发作期间受试者表达CRTh2的CD4+ T细胞的百分比更高，FEV1更差。随访期间，表达CRTh2的CD4+ T细胞%下降与肺功能改善相关。
结论：这些数据表明，外周血中的Th2细胞可能是哮喘急性发作症状增加的敏感指标，并可能成为哮喘急性发作的生物标志物。
Th2 cell markers in peripheral blood increase during an acute asthma exacerbation
Shrestha Palikhe N, Wu Y, Konrad E, Gandhi VD, Rowe BH, Vliagoftis H, Cameron L.
Allergy; 2021; Jan ;76(1):281-290.
Abstract
BACKGROUND: Allergic asthma is characterized by type 2 inflammation. We have shown the presence of increased type 2 inflammation in patients with severe asthma and those with frequent exacerbations. However, it is not known whether increased type 2 inflammation drives asthma exacerbations. This study aims to determine Th2 immune parameters in patients presenting to the emergency department (ED) with an acute asthma exacerbation and correlate these parameters with clinical and physiological measures of asthma.
METHODS: Sixteen adults presenting to the ED with acute asthma exacerbations were recruited after giving informed consent. Ten patients returned 2 weeks later for follow-up. Physiological parameters, asthma control (ACQ6), asthma quality of life (AQLQ) questionnaires, and venous blood were collected during both visits. An immune cell profiling was performed by whole blood flow cytometry: CD4+ T cells, Th2 cells (CD4+ CRTh2+ T cells and % of CD4+ T cells expressing CRTh2), eosinophils and innate lymphoid cells (ILC2).
RESULTS: During exacerbation, peripheral blood Th2 cell numbers correlated with ACQ6 and AQLQ scores, while ILC2 and eosinophil numbers did not. Subjects had higher % of CD4+ T cells expressing CRTh2 and worse FEV1 during exacerbation compared with the follow-up. The decrease in the % of CD4+ T cells expressing CRTh2 seen during the follow-up visit correlated with the improvement in lung function.
CONCLUSION: These data suggest that Th2 cells in peripheral blood may be a sensitive measure of increasing symptoms in patients with asthma exacerbations and may serve as a biomarker of an asthma exacerbation.
背景：过敏性哮喘的特征是2型炎症。我们已经表明，重症哮喘患者与频繁急性发作的患者存在2型炎症的增加。然而，目前尚不清楚2型炎症的增加是否会导致哮喘急性发作。本研究旨在确定因哮喘急性发作到急诊科(ED)就诊患者的Th2免疫参数，并将这些参数与哮喘的临床和生理指标联系起来。
方法：在给予知情同意后，招募16例因哮喘急性发作到急诊科(ED)就诊的成人哮喘患者。10例受试者2周后返回进行随访。在两次随访期间收集生理参数、哮喘控制问卷(ACQ6)、哮喘生活质量问卷(AQLQ)和静脉血。通过全血流式细胞术对免疫细胞进行分析：CD4+ T细胞、Th2细胞(CD4+ CRTh2+ T细胞和表达CRTh2的CD4+ T细胞%)、嗜酸性粒细胞和2型固有淋巴细胞(ILC2)。
结果：在急性发作期间，外周血Th2细胞数量与ACQ6和AQLQ评分相关，而与ILC2和嗜酸性粒细胞数量不相关。与随访期间相比，急性发作期间受试者表达CRTh2的CD4+ T细胞的百分比更高，FEV1更差。随访期间，表达CRTh2的CD4+ T细胞%下降与肺功能改善相关。
结论：这些数据表明，外周血中的Th2细胞可能是哮喘急性发作症状增加的敏感指标，并可能成为哮喘急性发作的生物标志物。
(邓稞1 张红萍1 王刚2 四川大学华西医院中西医结合科呼吸病组 610041 摘译)
（Allergy; 2021; Jan ;76(1):281-290.）
（Allergy; 2021; Jan ;76(1):281-290.）
Th2 cell markers in peripheral blood increase during an acute asthma exacerbation
Shrestha Palikhe N, Wu Y, Konrad E, Gandhi VD, Rowe BH, Vliagoftis H, Cameron L.
Allergy; 2021; Jan ;76(1):281-290.
Abstract
BACKGROUND: Allergic asthma is characterized by type 2 inflammation. We have shown the presence of increased type 2 inflammation in patients with severe asthma and those with frequent exacerbations. However, it is not known whether increased type 2 inflammation drives asthma exacerbations. This study aims to determine Th2 immune parameters in patients presenting to the emergency department (ED) with an acute asthma exacerbation and correlate these parameters with clinical and physiological measures of asthma.
METHODS: Sixteen adults presenting to the ED with acute asthma exacerbations were recruited after giving informed consent. Ten patients returned 2 weeks later for follow-up. Physiological parameters, asthma control (ACQ6), asthma quality of life (AQLQ) questionnaires, and venous blood were collected during both visits. An immune cell profiling was performed by whole blood flow cytometry: CD4+ T cells, Th2 cells (CD4+ CRTh2+ T cells and % of CD4+ T cells expressing CRTh2), eosinophils and innate lymphoid cells (ILC2).
RESULTS: During exacerbation, peripheral blood Th2 cell numbers correlated with ACQ6 and AQLQ scores, while ILC2 and eosinophil numbers did not. Subjects had higher % of CD4+ T cells expressing CRTh2 and worse FEV1 during exacerbation compared with the follow-up. The decrease in the % of CD4+ T cells expressing CRTh2 seen during the follow-up visit correlated with the improvement in lung function.
CONCLUSION: These data suggest that Th2 cells in peripheral blood may be a sensitive measure of increasing symptoms in patients with asthma exacerbations and may serve as a biomarker of an asthma exacerbation.
上一篇：
按需使用布地奈德福莫特罗治疗青少年轻度哮喘的有效性和安全性
下一篇：
奥马珠单抗妊娠登记和疾病匹配的对照队列中的妊娠结局
