T2型哮喘与特定的室内真菌菌落和微生物群有关
2020/10/14
摘要
背景：已有研究表明微生物环境暴露与哮喘之间的联系，但是没有一项研究将哮喘患者肺脏与室内微生物组的深度测序、肺功能、临床和内表型相结合。
目的：本研究旨在探讨室内微生物暴露与肺部微生物群落的关系，并记录微生物暴露对重症哮喘（SA）患者炎症和临床转归的影响。
方法：采用静电除尘器（EDCs）对55例来自全国COBRA队列的SA患者进行室内微生物区系分析。在这些患者中，有22例在稳定期或急性发作期产生痰，并收集和分析了完整的EDC和痰液样本。我们使用扩增子靶向宏基因组学方法，依据2型（T2）哮喘比较EDC和痰中的微生物群落。
结果：与低T2型SA患者相比，高T2型SA患者室内微生物区系细菌α多样性增加，真菌α多样性降低，后者与FeNO水平显著相关。T2型中，EDC真菌群落的β多样性显著聚集。此外，当病人出现急性加重时，痰标本和EDC样本中常见真菌类群的比例显著升高。
结论：这些结果首次说明了哮喘患者室内真菌群落与临床特征之间的潜在联系，这将有助于进一步研究哮喘患者室内环境、真菌和宿主之间的相互作用。
Type 2-high asthma is associated with a specific indoor mycobiome and microbiome
VANDENBORGHT Louise-Eva, Raphaël Enaud, Charlotte Urien, Noémie Coron, GIRODET Pierre-Olivier, Stéphanie Ferreira, Patrick Berger, Laurence Delhaes
Abstract
Background: The links between microbial environmental exposures and asthma are well documented, but no study has combined deep-sequencing results from pulmonary and indoor microbiomes of asthmatic patients with spirometry, clinical and endotype parameters.
Objective: The goal of this study was to investigate the links between indoor microbial exposures and pulmonary microbial communities and to document the role of microbial exposures on inflammatory and clinical outcomes of patients with severe asthma (SA).
Methods: Fifty-five SA patients from the national COBRA cohort were enrolled for analyzing their indoor microbial flora through the use of electrostatic dust collectors (EDCs). Among these patients, 22 were able to produce sputa during stable or pulmonary exacerbation periods and had complete pairs of EDC and sputum samples, both collected and analysed. We used amplicon targeted metagenomics to compare microbial communities from EDC and sputum samples of patients according to type 2 (T2)-asthma endotypes.
Results: Compared to patients with T2-low SA, patients with T2-high SA exhibited an increase in bacterial alpha-diversity and a decrease in fungal alpha-diversity of their indoor microbial floras, the latter being significantly correlated with FeNO levels. The beta-diversity of the EDC mycobiome significantly clustered according to T2 endotypes. Moreover, the proportion of fungal taxa in common between sputum and EDC samples was significantly higher when patients exhibited acute exacerbation.
Conclusion: These results illustrated, for the first time, a potential association between the indoor mycobiome and clinical features of SA patients, which should renew interest in deciphering the interactions between indoor environment, fungi, and host in asthma.
背景：已有研究表明微生物环境暴露与哮喘之间的联系，但是没有一项研究将哮喘患者肺脏与室内微生物组的深度测序、肺功能、临床和内表型相结合。
目的：本研究旨在探讨室内微生物暴露与肺部微生物群落的关系，并记录微生物暴露对重症哮喘（SA）患者炎症和临床转归的影响。
方法：采用静电除尘器（EDCs）对55例来自全国COBRA队列的SA患者进行室内微生物区系分析。在这些患者中，有22例在稳定期或急性发作期产生痰，并收集和分析了完整的EDC和痰液样本。我们使用扩增子靶向宏基因组学方法，依据2型（T2）哮喘比较EDC和痰中的微生物群落。
结果：与低T2型SA患者相比，高T2型SA患者室内微生物区系细菌α多样性增加，真菌α多样性降低，后者与FeNO水平显著相关。T2型中，EDC真菌群落的β多样性显著聚集。此外，当病人出现急性加重时，痰标本和EDC样本中常见真菌类群的比例显著升高。
结论：这些结果首次说明了哮喘患者室内真菌群落与临床特征之间的潜在联系，这将有助于进一步研究哮喘患者室内环境、真菌和宿主之间的相互作用。
(中日友好医院呼吸与危重症医学科 李红雯 摘译 林江涛 审校)
(J Allergy Clin Immunol. 2020 Sep 11; S0091-6749(20)31248-3.)
(J Allergy Clin Immunol. 2020 Sep 11; S0091-6749(20)31248-3.)
Type 2-high asthma is associated with a specific indoor mycobiome and microbiome
VANDENBORGHT Louise-Eva, Raphaël Enaud, Charlotte Urien, Noémie Coron, GIRODET Pierre-Olivier, Stéphanie Ferreira, Patrick Berger, Laurence Delhaes
Abstract
Background: The links between microbial environmental exposures and asthma are well documented, but no study has combined deep-sequencing results from pulmonary and indoor microbiomes of asthmatic patients with spirometry, clinical and endotype parameters.
Objective: The goal of this study was to investigate the links between indoor microbial exposures and pulmonary microbial communities and to document the role of microbial exposures on inflammatory and clinical outcomes of patients with severe asthma (SA).
Methods: Fifty-five SA patients from the national COBRA cohort were enrolled for analyzing their indoor microbial flora through the use of electrostatic dust collectors (EDCs). Among these patients, 22 were able to produce sputa during stable or pulmonary exacerbation periods and had complete pairs of EDC and sputum samples, both collected and analysed. We used amplicon targeted metagenomics to compare microbial communities from EDC and sputum samples of patients according to type 2 (T2)-asthma endotypes.
Results: Compared to patients with T2-low SA, patients with T2-high SA exhibited an increase in bacterial alpha-diversity and a decrease in fungal alpha-diversity of their indoor microbial floras, the latter being significantly correlated with FeNO levels. The beta-diversity of the EDC mycobiome significantly clustered according to T2 endotypes. Moreover, the proportion of fungal taxa in common between sputum and EDC samples was significantly higher when patients exhibited acute exacerbation.
Conclusion: These results illustrated, for the first time, a potential association between the indoor mycobiome and clinical features of SA patients, which should renew interest in deciphering the interactions between indoor environment, fungi, and host in asthma.
上一篇：
录音中的急性咳嗽特征能区分儿童常见的呼吸系统疾病么？一项比较性的前瞻研究
下一篇：
哮喘及哮喘药物治疗对COVID-19患者预后的影响
