过敏性气道反应时肺部造血细胞外RNAs和囊泡的增加
2020/04/07
摘要
在体外可以被许多类型细胞释放的细胞外RNAs（exRNAs），通常在囊泡内受到保护，并能改变受体细胞的功能。为了确定组织炎症过程中exRNAs和携带他们的胞外囊泡（EVs）的组成和细胞来源在体内是如何变化的，我们分析小鼠肺过敏原激发前后的支气管肺泡灌洗液（BALF）。在肺中，细胞外microRNAs（exmiRNAs）的组成与气道上皮细胞高度相关。通过细胞类型特异性膜标记和单囊泡流技术，我们还发现检测到的囊泡中80%是上皮细胞来源的。诱导过敏性气道炎症后，通过免疫细胞选择性表达的miRNAs（包括miR-223和miR-142a）增加，造血细胞来源的EVs也增加2倍以上。这些数据表明，浸润免疫细胞在炎症组织中释放出ex-miRNAs和EVs，以改变局部细胞的外环境。
Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses.
Pua HH, Happ HC, Gray CJ, Mar DJ, Chiou NT, Hesse LE, Ansel KM,
Abstract
Extracellular RNAs (exRNAs) can be released by numerous cell types in vitro, are often protected within vesicles, and can modify recipient cell function. To determine how the composition and cellular sources of exRNAs and the extracellular vesicles (EVs) that carry them change in vivo during tissue inflammation, we analyzed bronchoalveolar lavage fluid (BALF) from mice before and after lung allergen challenge. In the lung, extracellular microRNAs (ex-miRNAs) had a composition that was highly correlated with airway-lining epithelium. Using cell type-specific membrane tagging and single vesicle flow, we also found that 80% of detected vesicles were of epithelial origin. After the induction of allergic airway inflammation, miRNAs selectively expressed by immune cells, including miR-223 and miR-142a, increased and hematopoietic-cell-derived EVs also increased >2-fold. These data demonstrate that infiltrating immune cells release ex-miRNAs and EVs in inflamed tissues to alter the local extracellular environment.
在体外可以被许多类型细胞释放的细胞外RNAs（exRNAs），通常在囊泡内受到保护，并能改变受体细胞的功能。为了确定组织炎症过程中exRNAs和携带他们的胞外囊泡（EVs）的组成和细胞来源在体内是如何变化的，我们分析小鼠肺过敏原激发前后的支气管肺泡灌洗液（BALF）。在肺中，细胞外microRNAs（exmiRNAs）的组成与气道上皮细胞高度相关。通过细胞类型特异性膜标记和单囊泡流技术，我们还发现检测到的囊泡中80%是上皮细胞来源的。诱导过敏性气道炎症后，通过免疫细胞选择性表达的miRNAs（包括miR-223和miR-142a）增加，造血细胞来源的EVs也增加2倍以上。这些数据表明，浸润免疫细胞在炎症组织中释放出ex-miRNAs和EVs，以改变局部细胞的外环境。
(中日友好医院呼吸与危重症医学科 张清 摘译 林江涛 审校)
(Cell Rep 2019 01 22;26(4) DOI: 10.1016/j.celrep.2019.01.002 )
(Cell Rep 2019 01 22;26(4) DOI: 10.1016/j.celrep.2019.01.002 )
Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses.
Pua HH, Happ HC, Gray CJ, Mar DJ, Chiou NT, Hesse LE, Ansel KM,
Abstract
Extracellular RNAs (exRNAs) can be released by numerous cell types in vitro, are often protected within vesicles, and can modify recipient cell function. To determine how the composition and cellular sources of exRNAs and the extracellular vesicles (EVs) that carry them change in vivo during tissue inflammation, we analyzed bronchoalveolar lavage fluid (BALF) from mice before and after lung allergen challenge. In the lung, extracellular microRNAs (ex-miRNAs) had a composition that was highly correlated with airway-lining epithelium. Using cell type-specific membrane tagging and single vesicle flow, we also found that 80% of detected vesicles were of epithelial origin. After the induction of allergic airway inflammation, miRNAs selectively expressed by immune cells, including miR-223 and miR-142a, increased and hematopoietic-cell-derived EVs also increased >2-fold. These data demonstrate that infiltrating immune cells release ex-miRNAs and EVs in inflamed tissues to alter the local extracellular environment.
