健康和哮喘患者呼出生物标志物的昼夜节律
2019/07/11
摘要
背景：昼夜节律控制着身体中包括健康和疾病的许多生物过程。深入理解疾病相关生物标志物的日变化在临床实践应用中是非常重要的，并且需要有昼夜节律的生物标志物来进行慢性疾病的进一步研究。本文旨在明确健康和哮喘患者呼出气一氧化氮和呼吸挥发性生物标志物是否有日变化。
方法：10名中度特应性哮喘患者（常规吸入皮质类固醇激素）和10名健康受试者（均为非吸烟者）完成了整夜的随访，每6小时采集呼出气体挥发物和用力呼出气一氧化氮检测。挥发物吸附在吸附剂材料上用于热解吸后，使用气相色谱质谱法分析呼吸挥发物。
结果：九种呼吸挥发物（包括丙酮和异戊二烯）在所有受试者中表现出昼夜变化。此外，几种挥发物的昼夜节律模式在哮喘患者中出现改变和呼出气一氧化氮在哮喘中表现为节律性，而健康对照中则没有出现。
结论：昼夜节律的标记物可以在呼吸中识别，并且为通过昼夜分析以帮助治疗疾病提供了思路。这项工作表明在以后设计生物标志物发现研究时必须控制时间，但仍需要进行大型的队列研究来验证和扩展这些发现。
Circadian rhythm of exhaled biomarkers in health and asthma.
Wilkinson M, Maidstone R, Loudon A, Blaikley J, White IR, Singh D, Ray DW, Goodacre R, Fowler SJ, Durrington HJ.
Abstract
BACKGROUND:Circadian rhythms control many biological processes in the body in both health and disease. Greater understanding of diurnal variability in disease related biomarkers is crucial for their application in clinical practice and biomarkers of circadian rhythm are required to facilitate further research into disturbed chronicity. To determine if fractional exhaled nitric oxide and breath volatile biomarkers vary rhythmically during the day in healthy and asthmatic individuals.
METHODS:Ten individuals with moderate, atopic asthma (on regular inhaled corticosteroids) and 10 healthy volunteers (all non-smokers) completed an overnight visit where their exhaled breath volatiles and forced exhaled nitric oxide levels were collected every 6 h. Breath volatiles were analysed using gas chromatography mass spectrometry, after trapping these volatiles on sorbent materials for thermal desorption.
RESULTS:Nine breath volatiles (including acetone and isoprene) exhibit diurnal variation across all individuals. Furthermore the circadian pattern of several VOCs is altered in individuals with asthma and fractional exhaled nitric oxide is rhythmic in asthma but not in healthy controls.
CONCLUSIONS:Markers of circadian rhythm can be identified in breath and may offer insight into circadian profiling to help treat disease. Additionally this work suggests that time of day must be controlled when designing future biomarker discovery studies. Further work is required with larger cohorts to validate and extend these findings.
背景：昼夜节律控制着身体中包括健康和疾病的许多生物过程。深入理解疾病相关生物标志物的日变化在临床实践应用中是非常重要的，并且需要有昼夜节律的生物标志物来进行慢性疾病的进一步研究。本文旨在明确健康和哮喘患者呼出气一氧化氮和呼吸挥发性生物标志物是否有日变化。
方法：10名中度特应性哮喘患者（常规吸入皮质类固醇激素）和10名健康受试者（均为非吸烟者）完成了整夜的随访，每6小时采集呼出气体挥发物和用力呼出气一氧化氮检测。挥发物吸附在吸附剂材料上用于热解吸后，使用气相色谱质谱法分析呼吸挥发物。
结果：九种呼吸挥发物（包括丙酮和异戊二烯）在所有受试者中表现出昼夜变化。此外，几种挥发物的昼夜节律模式在哮喘患者中出现改变和呼出气一氧化氮在哮喘中表现为节律性，而健康对照中则没有出现。
结论：昼夜节律的标记物可以在呼吸中识别，并且为通过昼夜分析以帮助治疗疾病提供了思路。这项工作表明在以后设计生物标志物发现研究时必须控制时间，但仍需要进行大型的队列研究来验证和扩展这些发现。
(中日友好医院呼吸与危重症医学科 王瑞茵 摘译 林江涛 审校)
(Eur Respir J. 2019 Jun 20. pii: 1901068. doi: 10.1183/13993003.01068-2019. [Epub ahead of print])
(Eur Respir J. 2019 Jun 20. pii: 1901068. doi: 10.1183/13993003.01068-2019. [Epub ahead of print])
Circadian rhythm of exhaled biomarkers in health and asthma.
Wilkinson M, Maidstone R, Loudon A, Blaikley J, White IR, Singh D, Ray DW, Goodacre R, Fowler SJ, Durrington HJ.
Abstract
BACKGROUND:Circadian rhythms control many biological processes in the body in both health and disease. Greater understanding of diurnal variability in disease related biomarkers is crucial for their application in clinical practice and biomarkers of circadian rhythm are required to facilitate further research into disturbed chronicity. To determine if fractional exhaled nitric oxide and breath volatile biomarkers vary rhythmically during the day in healthy and asthmatic individuals.
METHODS:Ten individuals with moderate, atopic asthma (on regular inhaled corticosteroids) and 10 healthy volunteers (all non-smokers) completed an overnight visit where their exhaled breath volatiles and forced exhaled nitric oxide levels were collected every 6 h. Breath volatiles were analysed using gas chromatography mass spectrometry, after trapping these volatiles on sorbent materials for thermal desorption.
RESULTS:Nine breath volatiles (including acetone and isoprene) exhibit diurnal variation across all individuals. Furthermore the circadian pattern of several VOCs is altered in individuals with asthma and fractional exhaled nitric oxide is rhythmic in asthma but not in healthy controls.
CONCLUSIONS:Markers of circadian rhythm can be identified in breath and may offer insight into circadian profiling to help treat disease. Additionally this work suggests that time of day must be controlled when designing future biomarker discovery studies. Further work is required with larger cohorts to validate and extend these findings.
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连续测定工作及休息期间呼出气一氧化氮在职业性哮喘方面的诊断
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不同哮喘表型痰柱状上皮细胞及其产物的失调
