蒸煮过的干草对患重症哮喘的马临床症状及气道免疫反应的影响
2019/01/09
摘要
背景：避免抗原刺激可显著逆转患重症哮喘(sEA)的马的气道阻塞。到目前为止，还没有已发表的研究调查蒸煮过的干草对患重症哮喘的马的下呼吸道状况的影响。目的是明确对于患重症哮喘的马和对照组的马经过实验性暴露于蒸煮过的干草和干燥的干草后在临床、细胞学和细胞因子方面呼吸系统的反应。
结果：本研究采用6匹患重症哮喘的马和6匹对照组的马进行队列研究。第0天，两组连续喂蒸煮过的干草5天，经过26天的洗净期后再连续喂干燥的干草5天。调查研究每个过程前2天到结束后的5天之间包括临床评分、气管粘液积累、支气管肺泡灌洗液(BALF)细胞学和细胞因子mRNA表达的资料。饲喂蒸煮过的干草可以显著降低其霉菌含量(P < 0.001)。饲喂干燥的干草可以显著增加黏液评分(P = 0.01)。饲喂干草对临床评分无显著影响。饲喂干草（蒸煮过的和干燥的）均可以显著增加BALF中中性粒细胞百分比(P < 0.001)以及IL-1β(P = 0.024), IL-6R (P = 0.021), IL-18 (P = 0.009)和IL-23 (P = 0.036) mRNA的表达。BALF中IL-1β，IL-6R和IL-23相对mRNA的表达与中性粒细胞百分比和临床和气管黏液得分有显著相关性。
结论：蒸煮过程可以显著降低发霉量，但在饲喂干草时对患重症哮喘的马的呼吸系统的反应有不同程度的影响。基于BALF细胞学和细胞因子谱，其作为一种对患重症哮喘马的非药物治疗存在争议。
The influence of hay steaming on clinical signs and airway immune response in severe asthmatic horses.
Orard M, Hue E, Couroucé A, Bizon-Mercier C, Toquet MP, Moore-Colyer M, Couëtil L, Pronost S, Paillot R, Demoor M, Richard EA.
Abstract
BACKGROUND: Avoidance of antigenic stimuli was found to significantly reverse airway obstruction of horses with severe equine asthma (sEA). To date, no published study investigated the influence of steaming hay on lower airway condition of sEA-affected horses. The objectives were to determine the clinical, cytological and cytokine respiratory responses of both sEA and control (CTL) horses experimentally exposed to steamed or dry hay.
RESULTS: A cohort of 6 sEA horses and 6 CTL horses was involved in this field study. On day 0, both groups were fed with steamed hay for 5 consecutive days, followed by a wash-out period of 26 days prior to be fed with dry hay for 5 consecutive days. Investigations performed 2 days prior to and 5 days after each challenge included clinical score, tracheal mucus accumulation, and bronchoalveolar lavage fluid (BALF) cytology and cytokine mRNA expression. Feeding steamed hay significantly decreased its mould content (P < 0.001). Mucus score significantly increased when feeding dry hay (P = 0.01). No significant influence of challenge type was found on clinical score. Percentages of neutrophils (P < 0.001) as well as mRNA expression of IL-1β (P = 0.024), IL-6R (P = 0.021), IL-18 (P = 0.009) and IL-23 (P = 0.036) in BALF of sEA affected horses were significantly increased after both (steamed and dry hay) challenges. Relative mRNA expression of IL-1β, IL-6R and IL-23 in BALF were also significantly correlated to neutrophil percentages and both clinical and tracheal mucus score.
CONCLUSIONS: Steaming significantly decreased mould content but inconsistently influenced the respiratory response of sEA affected horses when fed hay. Based on BALF cytology and cytokine profiles, its relevance might be controversial as a non-medicinal therapy for sEA-affected horses.
背景：避免抗原刺激可显著逆转患重症哮喘(sEA)的马的气道阻塞。到目前为止，还没有已发表的研究调查蒸煮过的干草对患重症哮喘的马的下呼吸道状况的影响。目的是明确对于患重症哮喘的马和对照组的马经过实验性暴露于蒸煮过的干草和干燥的干草后在临床、细胞学和细胞因子方面呼吸系统的反应。
结果：本研究采用6匹患重症哮喘的马和6匹对照组的马进行队列研究。第0天，两组连续喂蒸煮过的干草5天，经过26天的洗净期后再连续喂干燥的干草5天。调查研究每个过程前2天到结束后的5天之间包括临床评分、气管粘液积累、支气管肺泡灌洗液(BALF)细胞学和细胞因子mRNA表达的资料。饲喂蒸煮过的干草可以显著降低其霉菌含量(P < 0.001)。饲喂干燥的干草可以显著增加黏液评分(P = 0.01)。饲喂干草对临床评分无显著影响。饲喂干草（蒸煮过的和干燥的）均可以显著增加BALF中中性粒细胞百分比(P < 0.001)以及IL-1β(P = 0.024), IL-6R (P = 0.021), IL-18 (P = 0.009)和IL-23 (P = 0.036) mRNA的表达。BALF中IL-1β，IL-6R和IL-23相对mRNA的表达与中性粒细胞百分比和临床和气管黏液得分有显著相关性。
结论：蒸煮过程可以显著降低发霉量，但在饲喂干草时对患重症哮喘的马的呼吸系统的反应有不同程度的影响。基于BALF细胞学和细胞因子谱，其作为一种对患重症哮喘马的非药物治疗存在争议。
(中日友好医院呼吸与危重症医学科 禹汶伯 摘译 林江涛 审校)
(BMC Vet Res. 2018 Nov 15;14(1):345.)
(BMC Vet Res. 2018 Nov 15;14(1):345.)
The influence of hay steaming on clinical signs and airway immune response in severe asthmatic horses.
Orard M, Hue E, Couroucé A, Bizon-Mercier C, Toquet MP, Moore-Colyer M, Couëtil L, Pronost S, Paillot R, Demoor M, Richard EA.
Abstract
BACKGROUND: Avoidance of antigenic stimuli was found to significantly reverse airway obstruction of horses with severe equine asthma (sEA). To date, no published study investigated the influence of steaming hay on lower airway condition of sEA-affected horses. The objectives were to determine the clinical, cytological and cytokine respiratory responses of both sEA and control (CTL) horses experimentally exposed to steamed or dry hay.
RESULTS: A cohort of 6 sEA horses and 6 CTL horses was involved in this field study. On day 0, both groups were fed with steamed hay for 5 consecutive days, followed by a wash-out period of 26 days prior to be fed with dry hay for 5 consecutive days. Investigations performed 2 days prior to and 5 days after each challenge included clinical score, tracheal mucus accumulation, and bronchoalveolar lavage fluid (BALF) cytology and cytokine mRNA expression. Feeding steamed hay significantly decreased its mould content (P < 0.001). Mucus score significantly increased when feeding dry hay (P = 0.01). No significant influence of challenge type was found on clinical score. Percentages of neutrophils (P < 0.001) as well as mRNA expression of IL-1β (P = 0.024), IL-6R (P = 0.021), IL-18 (P = 0.009) and IL-23 (P = 0.036) in BALF of sEA affected horses were significantly increased after both (steamed and dry hay) challenges. Relative mRNA expression of IL-1β, IL-6R and IL-23 in BALF were also significantly correlated to neutrophil percentages and both clinical and tracheal mucus score.
CONCLUSIONS: Steaming significantly decreased mould content but inconsistently influenced the respiratory response of sEA affected horses when fed hay. Based on BALF cytology and cytokine profiles, its relevance might be controversial as a non-medicinal therapy for sEA-affected horses.
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