对马被昆虫咬伤并发超敏反应、马严重哮喘或两种情况兼有时其血清中过敏原特异性免疫球蛋白E的表达的研究
2018/12/17
摘要
背景：遗传学、流行病学和临床研究证据表明，马发生过敏的表现可以同时发生。
目的：探讨马被昆虫咬伤并发超敏反应(IBH)同时患有严重哮喘(EA)是否比只有马被昆虫咬伤并发超敏反应（IBH）或者只有马严重哮喘（EA）过敏原特异性血清免疫球蛋白E (IgE)浓度和血清总IgE浓度更高。
研究动物：健康对照马(C, n = 40)、IBH马(IBH, n = 24)、重度EA马(EA, n = 18)、两种情况兼有马(IBH/EA, n = 23)。
方法：在我们的回顾性比较研究中，我们使用了患有严重EA、IBH和健康对照马的血清。采用酶联免疫吸附法测定15种重组(r)过敏原特异性IgE及血清总IgE浓度。
结果：IBH组(r-Culicoides IgE中值和:405 nm处的光密度[OD405] = 3.54[0.48-15.07])和IBH/EA组(OD405 = 4.55[0.46-17.15])显著高于健康对照组(OD405 = 0.44[0.21-2.05])和EA组(OD405 = 0.6[0.2-2.9])，差异有统计学意义(P < .001)。IBH组与IBH/EA组之间无显著差异(P > 0.05)。各组间其他r-过敏原及血清总IgE浓度差异无统计学意义。与对照组相比，患有严重IBH的马IgE浓度显著升高至5倍的r-Culicoides (P <0 .05)，而患有中度IBH的马IgE浓度显著升高至3倍的 r-Culicoides (P < 0.05)。
结论及临床意义：马由IBH发展为EA可能与IgE介导的免疫反应无关，与其他免疫病理机制有关。
Allergen-specific immunoglobulin E in sera of horses affected with insect bite hypersensitivity, severe equine asthma or both conditions.
Verdon M1, Lanz S1, Rhyner C2, Gerber V1, Marti E3.
Abstract
BACKGROUND: Genetic, epidemiologic, and clinical evidence suggests that, in horses, there are manifestations of hypersensitivity that can occur together.
OBJECTIVES: To investigate whether concurrent insect bite hypersensitivity (IBH) and severe equine asthma (EA) is associated with higher allergen-specific and total serum immunoglobulin E (IgE) concentrations than only EA or IBH.
ANIMALS: Healthy control horses (C, n = 40), horses with IBH (IBH, n = 24), severe EA (EA, n = 18), and both conditions (IBH/EA, n = 23) were included.
METHODS: In our retrospective comparative study, sera from horses with signs of severe EA, IBH, and control animals were used. IgE specific for 15 recombinant (r) allergens as well as total serum IgE concentrations were measured by enzyme-linked immunosorbent assay.
RESULTS: Group IBH (median sum r-Culicoides IgE: optical density at 405 nm [OD405 ] = 3.54 [0.48-15.07]) and group IBH/EA (OD405 = 4.55 [0.46-17.15]) had significantly (P < .001) higher IgE against Culicoides r-allergens than groups C (OD405 = 0.44 [0.21-2.05]) and EA (OD405 = 0.6 [0.2-2.9]). There were no significant (P > .05) differences between group IBH and group IBH/EA. No significant differences among the groups were found for the other r-allergens or total serum IgE concentration. Compared to controls, horses with severe IBH had significantly increased IgE concentration to 5 Culicoides r-allergens (P < .05), whereas horses with moderate IBH had significantly increased IgE concentration to only 3 Culicoides r-allergens (P < .05).
CONCLUSIONS AND CLINICAL IMPORTANCE: Susceptibility of IBH-affected horses to develop EA is likely not associated with IgE-mediated immune reactions but with other immunopathological mechanisms.
背景：遗传学、流行病学和临床研究证据表明，马发生过敏的表现可以同时发生。
目的：探讨马被昆虫咬伤并发超敏反应(IBH)同时患有严重哮喘(EA)是否比只有马被昆虫咬伤并发超敏反应（IBH）或者只有马严重哮喘（EA）过敏原特异性血清免疫球蛋白E (IgE)浓度和血清总IgE浓度更高。
研究动物：健康对照马(C, n = 40)、IBH马(IBH, n = 24)、重度EA马(EA, n = 18)、两种情况兼有马(IBH/EA, n = 23)。
方法：在我们的回顾性比较研究中，我们使用了患有严重EA、IBH和健康对照马的血清。采用酶联免疫吸附法测定15种重组(r)过敏原特异性IgE及血清总IgE浓度。
结果：IBH组(r-Culicoides IgE中值和:405 nm处的光密度[OD405] = 3.54[0.48-15.07])和IBH/EA组(OD405 = 4.55[0.46-17.15])显著高于健康对照组(OD405 = 0.44[0.21-2.05])和EA组(OD405 = 0.6[0.2-2.9])，差异有统计学意义(P < .001)。IBH组与IBH/EA组之间无显著差异(P > 0.05)。各组间其他r-过敏原及血清总IgE浓度差异无统计学意义。与对照组相比，患有严重IBH的马IgE浓度显著升高至5倍的r-Culicoides (P <0 .05)，而患有中度IBH的马IgE浓度显著升高至3倍的 r-Culicoides (P < 0.05)。
结论及临床意义：马由IBH发展为EA可能与IgE介导的免疫反应无关，与其他免疫病理机制有关。
(中日友好医院呼吸与危重症医学科 禹汶伯 摘译 林江涛 审校)
(J Vet Intern Med. 2018 Dec 6.)
(J Vet Intern Med. 2018 Dec 6.)
Allergen-specific immunoglobulin E in sera of horses affected with insect bite hypersensitivity, severe equine asthma or both conditions.
Verdon M1, Lanz S1, Rhyner C2, Gerber V1, Marti E3.
Abstract
BACKGROUND: Genetic, epidemiologic, and clinical evidence suggests that, in horses, there are manifestations of hypersensitivity that can occur together.
OBJECTIVES: To investigate whether concurrent insect bite hypersensitivity (IBH) and severe equine asthma (EA) is associated with higher allergen-specific and total serum immunoglobulin E (IgE) concentrations than only EA or IBH.
ANIMALS: Healthy control horses (C, n = 40), horses with IBH (IBH, n = 24), severe EA (EA, n = 18), and both conditions (IBH/EA, n = 23) were included.
METHODS: In our retrospective comparative study, sera from horses with signs of severe EA, IBH, and control animals were used. IgE specific for 15 recombinant (r) allergens as well as total serum IgE concentrations were measured by enzyme-linked immunosorbent assay.
RESULTS: Group IBH (median sum r-Culicoides IgE: optical density at 405 nm [OD405 ] = 3.54 [0.48-15.07]) and group IBH/EA (OD405 = 4.55 [0.46-17.15]) had significantly (P < .001) higher IgE against Culicoides r-allergens than groups C (OD405 = 0.44 [0.21-2.05]) and EA (OD405 = 0.6 [0.2-2.9]). There were no significant (P > .05) differences between group IBH and group IBH/EA. No significant differences among the groups were found for the other r-allergens or total serum IgE concentration. Compared to controls, horses with severe IBH had significantly increased IgE concentration to 5 Culicoides r-allergens (P < .05), whereas horses with moderate IBH had significantly increased IgE concentration to only 3 Culicoides r-allergens (P < .05).
CONCLUSIONS AND CLINICAL IMPORTANCE: Susceptibility of IBH-affected horses to develop EA is likely not associated with IgE-mediated immune reactions but with other immunopathological mechanisms.
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