毛喉鞘蕊花提取物通过调节炎症和细胞外基质缓解咳嗽和哮喘症状
2018/12/17
摘要
哮喘的特征是气道炎症浸润，气道炎症可导致气道重塑和气道高反应性。虽然毛喉鞘蕊花（CFK）已被用于治疗哮喘，但其所涉及的机制尚不清楚。为了探索毛喉鞘蕊花提取物（ECFK）的抗哮喘机制，本实验应用了磷酸组胺喷雾处理的豚鼠及卵清蛋白（OVA）致敏的大鼠。苏木精和伊红染色（H＆E）用于评估肺组织的病理变化。酶联免疫吸附测定（ELISA）用于测定血清和支气管肺泡灌洗液（BALF）中的细胞因子。免疫组织化学和Western印迹用于分析评估细胞间粘附分子-1（ICAM-1）、磷酸化p65（p-p65）、基质金属肽酶9（MMP-9）和金属蛋白酶组织抑制因子1（TIMP-1）的表达。经ECFK处理后，豚鼠的哮喘潜伏期显着延长。 H＆E结果显示，与对照组相比，12.8g / kg ECFK组中的嗜酸性粒细胞数显着降低。此外，ELISA结果表明，ECFK处理后血清和BALF中的白细胞介素（IL）-4、IL-5和IL-17显著降低，干扰素-γ（IFN-γ）和IL-10增加。此外，OVA致敏的大鼠中，ECFK处理导致肺组织中ICAM-1、p-p65、MMP-9和TIMP-1下调。综上，我们的研究结果表明，ECFK显著减轻了OVA诱导的哮喘炎症浸润和气道重塑。本研究为ECFK的临床应用奠定了理论基础。
Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix.
Ma C, Zou L, Xia Y, Tu Y, Xue D, Yang Y, Liu D, Liu Y, Wu H, Dan H, You P.
Abstract
Asthma is characterized by airway inflammatory infiltration, which leads to airway remodeling and airway hyperreactivity. Coleus forskohlii (CFK) has been used to treat asthma, however, the mechanism involved is not clear. To explore the antiasthma mechanism of extracts of Coleus forskohlii (ECFK), guinea pigs were administered with a spray of phosphoric acid histamine, and rats were sensitized with ovalbumin (OVA). Hematoxylin and eosin staining (H&E) were used to evaluate pathological changes in lung tissue. Enzyme-linked immunosorbent assay (ELISA) was used to determine cytokine levels in serum and bronchoalveolar lavage fluid (BALF). Immunohistochemistry and Western blot analysis were used to assess the expression of intercellular cell adhesion molecule-1 (ICAM-1), phosphorylation of p65 (p-p65), matrix metallopeptidase 9 (MMP-9), and tissue inhibitor of metalloproteinase 1 (TIMP-1). After ECFK treatment, the asthma incubation period of guinea pigs was significantly prolonged. The H&E results showed that the number of eosinophils in the 12.8 g/kg ECFK group was significantly lower when compared with the control group. Moreover, ELISA results demonstrated that interleukin (IL)-4, IL-5, and IL-17 in serum and BALF were significantly decreased, and interferon-γ (IFN-γ) and IL-10 were increased after ECFK treatment. In addition, ECFK treatment resulted in downregulation of ICAM-1, p-p65, MMP-9, and TIMP-1 in lung tissue after being sensitized by OVA. In conclusion, our findings indicated that ECFK significantly alleviated OVA-induced inflammatory infiltration and airway remodeling in asthma. This study laid a theoretical foundation for the clinical use of ECFK.
哮喘的特征是气道炎症浸润，气道炎症可导致气道重塑和气道高反应性。虽然毛喉鞘蕊花（CFK）已被用于治疗哮喘，但其所涉及的机制尚不清楚。为了探索毛喉鞘蕊花提取物（ECFK）的抗哮喘机制，本实验应用了磷酸组胺喷雾处理的豚鼠及卵清蛋白（OVA）致敏的大鼠。苏木精和伊红染色（H＆E）用于评估肺组织的病理变化。酶联免疫吸附测定（ELISA）用于测定血清和支气管肺泡灌洗液（BALF）中的细胞因子。免疫组织化学和Western印迹用于分析评估细胞间粘附分子-1（ICAM-1）、磷酸化p65（p-p65）、基质金属肽酶9（MMP-9）和金属蛋白酶组织抑制因子1（TIMP-1）的表达。经ECFK处理后，豚鼠的哮喘潜伏期显着延长。 H＆E结果显示，与对照组相比，12.8g / kg ECFK组中的嗜酸性粒细胞数显着降低。此外，ELISA结果表明，ECFK处理后血清和BALF中的白细胞介素（IL）-4、IL-5和IL-17显著降低，干扰素-γ（IFN-γ）和IL-10增加。此外，OVA致敏的大鼠中，ECFK处理导致肺组织中ICAM-1、p-p65、MMP-9和TIMP-1下调。综上，我们的研究结果表明，ECFK显著减轻了OVA诱导的哮喘炎症浸润和气道重塑。本研究为ECFK的临床应用奠定了理论基础。
(中日友好医院呼吸与危重症医学科 顾宪民 摘译 林江涛 审校)
(J Cell Biochem. 2018 Dec 5. doi: 10.1002/jcb.28243.)
(J Cell Biochem. 2018 Dec 5. doi: 10.1002/jcb.28243.)
Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix.
Ma C, Zou L, Xia Y, Tu Y, Xue D, Yang Y, Liu D, Liu Y, Wu H, Dan H, You P.
Abstract
Asthma is characterized by airway inflammatory infiltration, which leads to airway remodeling and airway hyperreactivity. Coleus forskohlii (CFK) has been used to treat asthma, however, the mechanism involved is not clear. To explore the antiasthma mechanism of extracts of Coleus forskohlii (ECFK), guinea pigs were administered with a spray of phosphoric acid histamine, and rats were sensitized with ovalbumin (OVA). Hematoxylin and eosin staining (H&E) were used to evaluate pathological changes in lung tissue. Enzyme-linked immunosorbent assay (ELISA) was used to determine cytokine levels in serum and bronchoalveolar lavage fluid (BALF). Immunohistochemistry and Western blot analysis were used to assess the expression of intercellular cell adhesion molecule-1 (ICAM-1), phosphorylation of p65 (p-p65), matrix metallopeptidase 9 (MMP-9), and tissue inhibitor of metalloproteinase 1 (TIMP-1). After ECFK treatment, the asthma incubation period of guinea pigs was significantly prolonged. The H&E results showed that the number of eosinophils in the 12.8 g/kg ECFK group was significantly lower when compared with the control group. Moreover, ELISA results demonstrated that interleukin (IL)-4, IL-5, and IL-17 in serum and BALF were significantly decreased, and interferon-γ (IFN-γ) and IL-10 were increased after ECFK treatment. In addition, ECFK treatment resulted in downregulation of ICAM-1, p-p65, MMP-9, and TIMP-1 in lung tissue after being sensitized by OVA. In conclusion, our findings indicated that ECFK significantly alleviated OVA-induced inflammatory infiltration and airway remodeling in asthma. This study laid a theoretical foundation for the clinical use of ECFK.
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对哮喘患者口服皮质类固醇激素不良事件和经济影响的一项系统综述
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Dupilumab在糖皮质激素依赖性重症哮喘中的疗效和安全性
