年轻人与老年支气管哮喘个体的上呼吸道微生物群及其功能基因差异
2018/11/13
摘要
背景：气道内的微生物菌群已被证实与哮喘的发病机制有关。上气道微生物菌群会对下气道微生物菌群失调产生影响。然而，到目前为止，上呼吸道微生物菌群组成对年轻人和老年人哮喘的影响还没有完全阐明。本文通过对年轻人和老年人上呼吸道微生物群落的宏基因组分析，确定其与成人哮喘的关系。
方法：分别从年轻和老年成人的哮喘患者和非哮喘患者中收集鼻咽拭子。通过高通量测序分析气道微生物群的组成和功能基因。
结果：年轻人和老年人的微生物组成存在差异，各年龄组哮喘患者和非哮喘患者的微生物组成亦存在差异。每个年龄组FEV1%与不同的细菌有关。在非哮喘患者中，与赖氨酸降解、N-聚糖生物合成、己内酰胺降解、PPAR信号通路相关的基因表达升高，这可能与炎症的减少和空气污染物的降解有关。在年轻哮喘患者中，与戊糖磷酸途径、脂多糖生物合成、鞭毛组装和细菌趋化有关的基因表达升高，这可能与炎症增加和致病菌定植有关。然而，老年患者气道微生物群的功能基因在哮喘发病上无显著差异。
结论：结果提示上气道微生物群的组成和功能可能影响哮喘的发病机制，不同年龄的微生物群可能发挥不同的作用。
（复旦大学附属中山医院呼吸与危重症医学科 魏婷婷 摘译 毕晶 审校）
（Allergy. 2018 Sep 22.）
Different Upper Airway Microbiome and Their Functional Genes Associated with Asthma in Young Adults and Elderly Individuals.
Lee JJ, et al. Allergy. 2018 Sep 22.
Abstract
BACKGROUND: Microbes in the airway has been shown to be associated with the pathogenesis of asthma. The upper airway microbiome influences the dysbiosis of the lower airway microbiome. However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated. Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
Methods:Nasopharyngeal swabs were collected from young-adult and elderly asthma patients and non-asthmatic subjects. The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing.
Results: The composition of microbiota differed between young-adult and elderly, and it was different between asthmatics and non-asthmatics in each age group. Different bacteria were related to FEV1 % predicted in each age group. Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction of inflammation and degradation of air pollutants, were higher in non-asthmatics. Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis – which may all be related to increased inflammation and colonization of pathogenic bacteria – were higher in young-adult asthmatic patients. However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
Conclusions: These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.
背景：气道内的微生物菌群已被证实与哮喘的发病机制有关。上气道微生物菌群会对下气道微生物菌群失调产生影响。然而，到目前为止，上呼吸道微生物菌群组成对年轻人和老年人哮喘的影响还没有完全阐明。本文通过对年轻人和老年人上呼吸道微生物群落的宏基因组分析，确定其与成人哮喘的关系。
方法：分别从年轻和老年成人的哮喘患者和非哮喘患者中收集鼻咽拭子。通过高通量测序分析气道微生物群的组成和功能基因。
结果：年轻人和老年人的微生物组成存在差异，各年龄组哮喘患者和非哮喘患者的微生物组成亦存在差异。每个年龄组FEV1%与不同的细菌有关。在非哮喘患者中，与赖氨酸降解、N-聚糖生物合成、己内酰胺降解、PPAR信号通路相关的基因表达升高，这可能与炎症的减少和空气污染物的降解有关。在年轻哮喘患者中，与戊糖磷酸途径、脂多糖生物合成、鞭毛组装和细菌趋化有关的基因表达升高，这可能与炎症增加和致病菌定植有关。然而，老年患者气道微生物群的功能基因在哮喘发病上无显著差异。
结论：结果提示上气道微生物群的组成和功能可能影响哮喘的发病机制，不同年龄的微生物群可能发挥不同的作用。
（复旦大学附属中山医院呼吸与危重症医学科 魏婷婷 摘译 毕晶 审校）
（Allergy. 2018 Sep 22.）
Different Upper Airway Microbiome and Their Functional Genes Associated with Asthma in Young Adults and Elderly Individuals.
Lee JJ, et al. Allergy. 2018 Sep 22.
Abstract
BACKGROUND: Microbes in the airway has been shown to be associated with the pathogenesis of asthma. The upper airway microbiome influences the dysbiosis of the lower airway microbiome. However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated. Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
Methods:Nasopharyngeal swabs were collected from young-adult and elderly asthma patients and non-asthmatic subjects. The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing.
Results: The composition of microbiota differed between young-adult and elderly, and it was different between asthmatics and non-asthmatics in each age group. Different bacteria were related to FEV1 % predicted in each age group. Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction of inflammation and degradation of air pollutants, were higher in non-asthmatics. Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis – which may all be related to increased inflammation and colonization of pathogenic bacteria – were higher in young-adult asthmatic patients. However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
Conclusions: These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.
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在混合炎症性哮喘小鼠模型中，中性粒细胞可通过中性粒细胞弹性蛋白酶介导的FGF-2诱导平滑肌增生
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哮喘患者的肺血管系统修剪：重症哮喘研究计划（SARP）队列
