年轻人与老年人不同上呼吸道微生物菌群及其哮喘相关功能基因
2018/10/23
摘要
背景：气道中的微生物已被证明与哮喘的发病机制有关。上呼吸道微生物菌群影响下呼吸道微生物菌群的稳态。然而，迄今为止，上呼吸道微生物菌群对成人和老年人哮喘的影响尚未完全阐明。本文分析了年轻人和老年人上呼吸道微生物菌群的宏基因组，以确定它们与成人哮喘的关系。
方法：从年轻人和老年哮喘患者和非哮喘患者收集鼻咽拭子。通过高通量测序分析气道微生物菌群的组成和功能基因。
结果：微生物群的组成在年轻人和老年人之间不同，并且在每个年龄组中哮喘患者和非哮喘患者之间存在差异。不同细菌与每个年龄组FEV1％预测值相关。与赖氨酸降解、N-聚糖生物合成、己内酰胺降解和PPAR信号通路（可能与炎症的减少和空气污染物的降解有关）相关的基因在非哮喘患者中较高。与磷酸戊糖途径、脂多糖生物合成、鞭毛组装和细菌趋化相关的基因（可能都与致病细菌的炎症和定植增加有关）在年轻成人哮喘患者中更高。然而，根据哮喘发病率，老年患者气道微生物菌群功能基因无显著差异。
结论：这些结果表明，上呼吸道微生物菌群的组成和功能可以影响哮喘发病机制，并且微生物菌群在不同年龄组发挥多种作用。本文受版权保护。
Different Upper Airway Microbiome and Their Functional Genes Associated with Asthma in Young Adults and Elderly Individuals.
Lee JJ, Kim SH, Lee MJ, Kim BK, Song WJ, Park HW, Cho SH, Hong SJ, Chang YS, Kim BS.
Abstract
BACKGROUND:Microbes in the airway has been shown to be associated with the pathogenesis of asthma. The upper airway microbiome influences the dysbiosis of the lower airway microbiome. However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated. Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
METHODS:Nasopharyngeal swabs were collected from young-adult and elderly asthma patients and non-asthmatic subjects. The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing.
RESULTS:The composition of microbiota differed between young-adult and elderly, and it was different between asthmatics and non-asthmatics in each age group. Different bacteria were related to FEV1%predicted in each age group. Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction of inflammation and degradation of air pollutants, were higher in non-asthmatics. Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis - which may all be related to increased inflammation and colonization of pathogenic bacteria - were higher in young-adult asthmatic patients. However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
CONCLUSIONS:These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group. This article is protected by copyright. All rights reserved.
背景：气道中的微生物已被证明与哮喘的发病机制有关。上呼吸道微生物菌群影响下呼吸道微生物菌群的稳态。然而，迄今为止，上呼吸道微生物菌群对成人和老年人哮喘的影响尚未完全阐明。本文分析了年轻人和老年人上呼吸道微生物菌群的宏基因组，以确定它们与成人哮喘的关系。
方法：从年轻人和老年哮喘患者和非哮喘患者收集鼻咽拭子。通过高通量测序分析气道微生物菌群的组成和功能基因。
结果：微生物群的组成在年轻人和老年人之间不同，并且在每个年龄组中哮喘患者和非哮喘患者之间存在差异。不同细菌与每个年龄组FEV1％预测值相关。与赖氨酸降解、N-聚糖生物合成、己内酰胺降解和PPAR信号通路（可能与炎症的减少和空气污染物的降解有关）相关的基因在非哮喘患者中较高。与磷酸戊糖途径、脂多糖生物合成、鞭毛组装和细菌趋化相关的基因（可能都与致病细菌的炎症和定植增加有关）在年轻成人哮喘患者中更高。然而，根据哮喘发病率，老年患者气道微生物菌群功能基因无显著差异。
结论：这些结果表明，上呼吸道微生物菌群的组成和功能可以影响哮喘发病机制，并且微生物菌群在不同年龄组发挥多种作用。本文受版权保护。
(中日友好医院呼吸与危重症医学科 顾宪民 摘译 林江涛 审校)
(Allergy. 2018 Sep 22. doi: 10.1111/all.13608.)
(Allergy. 2018 Sep 22. doi: 10.1111/all.13608.)
Different Upper Airway Microbiome and Their Functional Genes Associated with Asthma in Young Adults and Elderly Individuals.
Lee JJ, Kim SH, Lee MJ, Kim BK, Song WJ, Park HW, Cho SH, Hong SJ, Chang YS, Kim BS.
Abstract
BACKGROUND:Microbes in the airway has been shown to be associated with the pathogenesis of asthma. The upper airway microbiome influences the dysbiosis of the lower airway microbiome. However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated. Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma.
METHODS:Nasopharyngeal swabs were collected from young-adult and elderly asthma patients and non-asthmatic subjects. The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing.
RESULTS:The composition of microbiota differed between young-adult and elderly, and it was different between asthmatics and non-asthmatics in each age group. Different bacteria were related to FEV1%predicted in each age group. Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction of inflammation and degradation of air pollutants, were higher in non-asthmatics. Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis - which may all be related to increased inflammation and colonization of pathogenic bacteria - were higher in young-adult asthmatic patients. However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity.
CONCLUSIONS:These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group. This article is protected by copyright. All rights reserved.
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