MUC5AC和MUC5B糖型改变急性哮喘儿童患者的黏蛋白合成
2017/08/08
摘要
背景：弥漫性气道粘液阻塞是严重和致死哮喘的一个重要特点。MUC5AC和 MUC5B是发现于气道粘液中的主要凝胶形成黏蛋白。气道粘液中的黏蛋白合成可能影响其功能性质。
方法：我们用蛋白质印迹法对年龄匹配的急性和稳定性儿童哮喘患者和健康对照组的痰中的主要气道黏蛋白MUC5AC和MUC5B进行量化比较。
结果：收集了38名儿童（13名急性哮喘患者，15名稳定性哮喘患者和10名健康对照者）的痰标本。健康对照者的痰中MUC5AC浓度为7.6 μg/ml，稳定性哮喘患者的痰中MUC5AC浓度为22.4 μg/ml(p=0.17)，急性哮喘患者的痰中MUC5AC浓度为44.7 μg/ml(p<0.05)。MUC5B的浓度变化更小，健康对照者、稳定性哮喘患者和急性哮喘患者的浓度分别为156.2 μg/ml, 222.3 μg/ml和254.8 μg/ml。急性哮喘患者的MUC5AC浓度越大，健康对照者与急性哮喘患者的MUC5B:MUC5AC率变化越显著（p<0.05）。组间的MUC5B糖型有显著差异，只在急性哮喘患者中被发现。
结论：MUC5AC的增加和MUC5B糖型的出现显著改变了急性哮喘儿童患者的黏蛋白合成。这些变化可能是急性哮喘患者气道粘液阻塞的重要影响因素。
MUC5AC and a glycosylated variant of MUC5B alter mucin composition in children with acute asthma.
Welsh KG1, Rousseau K2, Fisher G1, Bonser LR2, Bradding P3, Brightling CE3, Thornton DJ4, Gaillard EA5.
Author information
Abstract
BACKGROUND:Diffuse airway mucus obstruction is an important feature of severe and fatal asthma. MUC5AC and MUC5B are the principal gel-forming mucins found in airway mucus. The mucin composition of airway mucus likely affects its functional properties.
METHODS:We quantified the principal airway mucins MUC5AC and MUC5B in the sputum of age-matched children with acute and stable asthma and healthy controls using Western blotting.
RESULTS:Sputum samples from 38 children (13 acute asthma, 15 stable asthma, 10 controls) were obtained. Sputum MUC5AC concentrations were 7.6 μg/ml in controls, 22.4 μg/ml in stable asthma (p=0.17) and 44.7 μg/ml in acute asthma (p<0.05). Concentration of MUC5B showed less variation with, 156.2 μg/ml, 222.3 μg/ml and 254.8 μg/ml in healthy controls, stable and acute asthma respectively. The greater MUC5AC concentration in acute asthma resulted in a significantly altered MUC5B:MUC5AC ratio between healthy control and acute asthma (p<0.05). Significant differences in MUC5B glycoforms were present between the groups, with the low-charge-only glycoform being uniquely found in acute asthma.
CONCLUSIONS:Increased MUC5AC and the presence of a low-charge-only MUC5B glycoform significantly alters mucin composition in children with acute asthma. These changes may be important contributory factors to the airway mucus obstruction observed during acute asthma.
背景：弥漫性气道粘液阻塞是严重和致死哮喘的一个重要特点。MUC5AC和 MUC5B是发现于气道粘液中的主要凝胶形成黏蛋白。气道粘液中的黏蛋白合成可能影响其功能性质。
方法：我们用蛋白质印迹法对年龄匹配的急性和稳定性儿童哮喘患者和健康对照组的痰中的主要气道黏蛋白MUC5AC和MUC5B进行量化比较。
结果：收集了38名儿童（13名急性哮喘患者，15名稳定性哮喘患者和10名健康对照者）的痰标本。健康对照者的痰中MUC5AC浓度为7.6 μg/ml，稳定性哮喘患者的痰中MUC5AC浓度为22.4 μg/ml(p=0.17)，急性哮喘患者的痰中MUC5AC浓度为44.7 μg/ml(p<0.05)。MUC5B的浓度变化更小，健康对照者、稳定性哮喘患者和急性哮喘患者的浓度分别为156.2 μg/ml, 222.3 μg/ml和254.8 μg/ml。急性哮喘患者的MUC5AC浓度越大，健康对照者与急性哮喘患者的MUC5B:MUC5AC率变化越显著（p<0.05）。组间的MUC5B糖型有显著差异，只在急性哮喘患者中被发现。
结论：MUC5AC的增加和MUC5B糖型的出现显著改变了急性哮喘儿童患者的黏蛋白合成。这些变化可能是急性哮喘患者气道粘液阻塞的重要影响因素。
（中日友好医院呼吸与危重症医学科 禹汶伯 摘译 林江涛 审校）
（Chest. 2017 Jul 14. pii: S00123692(17)312424.doi:10.1016/j.chest.2017.07.001. [Epub ahead of print]）
（Chest. 2017 Jul 14. pii: S00123692(17)312424.doi:10.1016/j.chest.2017.07.001. [Epub ahead of print]）
MUC5AC and a glycosylated variant of MUC5B alter mucin composition in children with acute asthma.
Welsh KG1, Rousseau K2, Fisher G1, Bonser LR2, Bradding P3, Brightling CE3, Thornton DJ4, Gaillard EA5.
Author information
Abstract
BACKGROUND:Diffuse airway mucus obstruction is an important feature of severe and fatal asthma. MUC5AC and MUC5B are the principal gel-forming mucins found in airway mucus. The mucin composition of airway mucus likely affects its functional properties.
METHODS:We quantified the principal airway mucins MUC5AC and MUC5B in the sputum of age-matched children with acute and stable asthma and healthy controls using Western blotting.
RESULTS:Sputum samples from 38 children (13 acute asthma, 15 stable asthma, 10 controls) were obtained. Sputum MUC5AC concentrations were 7.6 μg/ml in controls, 22.4 μg/ml in stable asthma (p=0.17) and 44.7 μg/ml in acute asthma (p<0.05). Concentration of MUC5B showed less variation with, 156.2 μg/ml, 222.3 μg/ml and 254.8 μg/ml in healthy controls, stable and acute asthma respectively. The greater MUC5AC concentration in acute asthma resulted in a significantly altered MUC5B:MUC5AC ratio between healthy control and acute asthma (p<0.05). Significant differences in MUC5B glycoforms were present between the groups, with the low-charge-only glycoform being uniquely found in acute asthma.
CONCLUSIONS:Increased MUC5AC and the presence of a low-charge-only MUC5B glycoform significantly alters mucin composition in children with acute asthma. These changes may be important contributory factors to the airway mucus obstruction observed during acute asthma.
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