不同程度哮喘患者白细胞和血清中CD48的变化
2017/08/08
摘要
背景：CD48是嗜酸性粒细胞、肥大细胞上的一种膜受体（mCD48），以可溶性形式存在（sCD48）。CD48 在小鼠哮喘和通过其配体 CD244 促使肥大细胞和嗜酸性粒细胞的炎症相互作用中起关键作用。因此，CD48可能在人类哮喘中起着重要作用。
方法：两个独立队列研究（以色列和英国）评估了轻、中、重度哮喘和健康志愿者血液中白细胞mCD48表达和血清中sCD48情况。哮喘患者支气管活检行免疫组化CD48，评价sCD48对CD244依赖嗜酸性细胞活化的影响。
结果:嗜酸性粒细胞mCD48在中度哮喘患者中高表达而在重症哮喘中出现低表达。B淋巴细胞、T淋巴细胞、NK细胞和单核细胞的mCD48在重症哮喘中出现高表达。sCD48在轻度哮喘患者中增加，在重症哮喘患者中降低。sCD48 区分哮喘与健康的最佳临界值被确定为 > 1482 pg/ml (以色列) 和 > 1619 pg/ml (英国)。在哮喘患者支气管活检中，mCD48主要由嗜酸性粒细胞表达。sCD48抑制抗CD244引导的嗜酸性粒细胞活化。
结论：mCD48和sCD48在不同严重程度的哮喘患者外周血中的表达不同。sCD48抑制了CD244介导的嗜酸性粒细胞活化。这些研究发现提示了CD48在人类哮喘中可能起着重要作用。
CD48 on blood leukocytes and in serum of asthma patients varies with severity.
Gangwar RS1, Minai-Fleminger Y1, Seaf M1, Gutgold A1, Shikotra A2, Barber C3,4, Chauhan A5, Holgate S3,4, Bradding P2, Howarth P3,4, Eliashar R6, Berkman N7, Levi-Schaffer F1..
Abstract
BACKGROUND:CD48 is a membrane receptor (mCD48) on eosinophils and mast cells and exists in a soluble form (sCD48). CD48 has a pivotal role in murine asthma and in the proinflammatory interactions of mast cells with eosinophils via its ligand CD244. Thus, CD48 might be important in human asthma.
METHODS:Therefore, two separate cohorts (IL and UK) comprising mild, moderate, and severe asthma and healthy volunteers were evaluated for blood leukocyte mCD48 expression and sCD48 in serum. Asthmatic bronchial biopsies were immunostained for CD48. sCD48 effect on CD244-dependent eosinophil activation was evaluated.
RESULTS: Eosinophil mCD48 expression was significantly elevated in moderate while downregulated in severe asthma. mCD48 expression on B, T, and NK cells and monocytes in severe asthma was significantly increased. sCD48 levels were significantly higher in mild while reduced in severe asthma. sCD48 optimal cutoff values for differentiating asthma from health were identified as >1482 pg/ml (IL) and >1619 pg/ml (UK). In asthmatic bronchial biopsies, mCD48 was expressed predominantly by eosinophils. sCD48 inhibited anti-CD244-induced eosinophil activation.
CONCLUSIONS:mCD48 and sCD48 are differentially expressed in the peripheral blood of asthma patients of varying severity. sCD48 inhibits CD244-mediated eosinophil activation. These findings suggest that CD48 may play an important role in human asthma.
背景：CD48是嗜酸性粒细胞、肥大细胞上的一种膜受体（mCD48），以可溶性形式存在（sCD48）。CD48 在小鼠哮喘和通过其配体 CD244 促使肥大细胞和嗜酸性粒细胞的炎症相互作用中起关键作用。因此，CD48可能在人类哮喘中起着重要作用。
方法：两个独立队列研究（以色列和英国）评估了轻、中、重度哮喘和健康志愿者血液中白细胞mCD48表达和血清中sCD48情况。哮喘患者支气管活检行免疫组化CD48，评价sCD48对CD244依赖嗜酸性细胞活化的影响。
结果:嗜酸性粒细胞mCD48在中度哮喘患者中高表达而在重症哮喘中出现低表达。B淋巴细胞、T淋巴细胞、NK细胞和单核细胞的mCD48在重症哮喘中出现高表达。sCD48在轻度哮喘患者中增加，在重症哮喘患者中降低。sCD48 区分哮喘与健康的最佳临界值被确定为 > 1482 pg/ml (以色列) 和 > 1619 pg/ml (英国)。在哮喘患者支气管活检中，mCD48主要由嗜酸性粒细胞表达。sCD48抑制抗CD244引导的嗜酸性粒细胞活化。
结论：mCD48和sCD48在不同严重程度的哮喘患者外周血中的表达不同。sCD48抑制了CD244介导的嗜酸性粒细胞活化。这些研究发现提示了CD48在人类哮喘中可能起着重要作用。
(中日友好医院呼吸与危重症医学科 王瑞茵 摘译 林江涛 审校)
(Allergy. 2017 Jun;72(6):888-895. doi: 10.1111/all.13082. Epub 2016 Dec 1)
(Allergy. 2017 Jun;72(6):888-895. doi: 10.1111/all.13082. Epub 2016 Dec 1)
CD48 on blood leukocytes and in serum of asthma patients varies with severity.
Gangwar RS1, Minai-Fleminger Y1, Seaf M1, Gutgold A1, Shikotra A2, Barber C3,4, Chauhan A5, Holgate S3,4, Bradding P2, Howarth P3,4, Eliashar R6, Berkman N7, Levi-Schaffer F1..
Abstract
BACKGROUND:CD48 is a membrane receptor (mCD48) on eosinophils and mast cells and exists in a soluble form (sCD48). CD48 has a pivotal role in murine asthma and in the proinflammatory interactions of mast cells with eosinophils via its ligand CD244. Thus, CD48 might be important in human asthma.
METHODS:Therefore, two separate cohorts (IL and UK) comprising mild, moderate, and severe asthma and healthy volunteers were evaluated for blood leukocyte mCD48 expression and sCD48 in serum. Asthmatic bronchial biopsies were immunostained for CD48. sCD48 effect on CD244-dependent eosinophil activation was evaluated.
RESULTS: Eosinophil mCD48 expression was significantly elevated in moderate while downregulated in severe asthma. mCD48 expression on B, T, and NK cells and monocytes in severe asthma was significantly increased. sCD48 levels were significantly higher in mild while reduced in severe asthma. sCD48 optimal cutoff values for differentiating asthma from health were identified as >1482 pg/ml (IL) and >1619 pg/ml (UK). In asthmatic bronchial biopsies, mCD48 was expressed predominantly by eosinophils. sCD48 inhibited anti-CD244-induced eosinophil activation.
CONCLUSIONS:mCD48 and sCD48 are differentially expressed in the peripheral blood of asthma patients of varying severity. sCD48 inhibits CD244-mediated eosinophil activation. These findings suggest that CD48 may play an important role in human asthma.
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