对花生的实验食物过敏增强了小鼠气道对屋尘螨的免疫应答
2016/10/09
摘要
背景：食物过敏与过敏性哮喘的发病风险增加有关。哮喘是发生食物过敏的一个危险因素。对不同的身体部位的不同过敏原的免疫的相互作用可能参与其中。
目的：评估在灌胃花生（PE）致敏和屋尘螨（HDM）气道致敏之间免疫的相互作用
方法：BALB/c小鼠对胃内花生过敏或假性过敏，并激发了对花生的系统性反应。在致敏和激发之间，小鼠鼻内注入屋尘螨提取物或注入PBS作为对照。对屋尘螨的反应（嗜酸性粒细胞的募集，细胞因子反应，屋尘螨特异性免疫球蛋白，和气道高反应性）和对花生的反应（细胞因子反应、肠内肥大细胞、血清中的mMCP-1、体温）进行评估。
结果：提前进行花生致敏可增加在肺引流淋巴结中屋尘螨诱导产生的IL-4、IL-5、IL-13、IFNγ和屋尘螨致敏的小鼠中总IgE水平。然而，呼吸道炎性细胞的募集和气道高反应性在花生和屋尘螨双重致敏小鼠中没有恶化。另外，屋尘螨诱导的气道炎症没有显著影响免疫反应或者花生激发的系统性过敏反应。
结论和临床实用性：我们的数据显示，前期进行花生致敏可提高在小鼠气道中的IgE和屋尘螨致敏特异性的Th2反应。它有助于对过敏个体多致敏潜在免疫机制的理解。
（苏欣 审校）
Clin Exp Allergy. 2016 Aug 17. doi: 10.1111/cea.12799. [Epub ahead of print]
Experimental food allergy to peanut enhances the immune response to house dust mite in the airways of mice.
Utsch L1, Logiantara A1, van Ree R1,2, van Rijt LS1.
Author information
Abstract
BACKGROUND:Food allergy has been associated with an increased risk for the development of allergic asthma. Asthma is a risk factor for the development of an anaphylactic response to food allergens. An immunological interplay between sensitization to different allergens in different compartments of the body might be involved.
OBJECTIVE:To evaluate the immunological interplay between intragastrical peanut (PE) sensitization and respiratory sensitization to house dust mite (HDM) allergens.
METHODS:BALB/c mice were intra-gastrically sensitized to peanut or sham-sensitized and challenged systemically to PE. Between sensitization and challenge, mice were intranasally exposed to HDM extract or PBS, as a control. The response to HDM (eosinophil recruitment, cytokine response, HDM specific immunoglobulins, and airway hyperreactivity) and to PE (cytokine response, mast cells in gut, mMCP-1 in serum, body temperature) was assessed.
RESULTS:A preceding PE sensitization increased HDM induced production of IL-4, IL-5, IL-13 and IFNγ in lung draining lymph nodes and total IgE levels in HDM sensitized mice. However, recruitment of inflammatory cells to the airways or airway hyperreactivity was not aggravated in PE/HDM double-sensitized mice. Alternatively, HDM induced airway inflammation did not significantly affect the immune response or the anaphylactic response to a systemic challenge with peanut.
CONCLUSION AND CLINICAL RELEVANCE:Our data show that a preceding peanut sensitization boosted IgE and HDM-specific Th2 response in the airways in mice. It contributes to the understanding of the underlying immunological mechanism of polysensitization which often occurs in allergic individuals over time. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:HDM; food allergy; peanut allergy
Clin Exp Allergy. 2016 Aug 17. doi: 10.1111/cea.12799. [Epub ahead of print]
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