在过敏性哮喘和鼻炎小鼠模型中的慢性超重独立效应

2016/08/31

   摘要
   我们的目的是评估在过敏性哮喘和鼻炎的小鼠模型慢性超重的影响。40只BALB/c小鼠随机分为:A组(n = 10,对照)致敏、用生理盐水处理,B组(n = 10,哮喘)腹腔和鼻腔卵清蛋白处理来诱导过敏性哮喘和鼻炎, C组(n = 10,哮喘/旋转对照)和D(n = 10,哮喘/超重),在哮喘/鼻炎诱导的同时在正常重力下(1G)或超重(5g)旋转4周。与B组和C组相比,D组在BAL液中嗜酸性粒细胞、嗜中性粒细胞和淋巴细胞显著降低(P<0.05=。使用肺组织匀浆进行的RT-PCR中,D组IL-1β表达显著上调(P<0.001=,IL-4和IL-10的表达显著下调(P<0.05=。炎症细胞进入肺实质和鼻甲浸润,和呼吸上皮的厚度在D组均显著降低(P<0.05=。在D组,Bcl-2蛋白和血红素加氧酶-1的表达明显下调,Bax和胞外岐化酶的表达明显上调。因此,慢性超重可能通过参与抗氧化和促凋亡通路的基因调控对过敏性哮喘和鼻炎有独立效应。用超重机械治疗过敏性呼吸系统疾病是有可能的。
 
 
(杨冬 审校)
Sci Rep. 2016 Jun 2;6:27260. doi: 10.1038/srep27260.

 
 
 
Hormetic Effect of Chronic Hypergravity in a Mouse Model of Allergic Asthma and Rhinitis.
 
 
Jang TY1, Jung AY1, Kim YH1.
Author information

 
Abstract
We aimed to evaluate the effect of chronic hypergravity in a mouse model of allergic asthma and rhinitis. Forty BALB/c mice were divided as: group A (n = 10, control) sensitized and challenged with saline, group B (n = 10, asthma) challenged by intraperitoneal and intranasal ovalbumin (OVA) to induce allergic asthma and rhinitis, and groups C (n = 10, asthma/rotatory control) and D (n = 10, asthma/hypergravity) exposed to 4 weeks of rotation with normogravity (1G) or hypergravity (5G) during induction of asthma/rhinitis. Group D showed significantly decreased eosinophils, neutrophils, and lymphocytes in their BAL fluid compared with groups B and C (p < 0.05). In real-time polymerase chain reaction using lung homogenate, the expression of IL-1β was significantly upregulated (p < 0.001) and IL-4 and IL-10 significantly downregulated (p < 0.05) in group D. Infiltration of inflammatory cells into lung parenchyma and turbinate, and the thickness of respiratory epithelium was significantly reduced in group D (p < 0.05). The expression of Bcl-2 and heme oxygenase-1 were significantly downregulated, Bax and extracellular dismutase significantly upregulated in Group D. Therefore, chronic hypergravity could have a hormetic effect for allergic asthma and rhinitis via regulation of genes involved in antioxidative and proapoptotic pathways. It is possible that we could use hypergravity machinery for treating allergic respiratory disorders.

 
Sci Rep. 2016 Jun 2;6:27260. doi: 10.1038/srep27260.
 


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