暴露于高浓度内毒素可增加实验室动物工作者喘息的患病率：一项横断面研究
2016/07/28
摘要
背景:革兰阴性细菌的内毒素被发现在处理小动物的实验室和动物房的灰尘里和地面上有不同浓度
方法：横断面研究在两所大学的工作场所进行。粉尘样品从实验室和养大鼠、小鼠、豚鼠、地鼠兔的动物房收集，并用“鲎试剂”进行分析。也对没有动物的场所进行取样。对工作场所发现的内毒素浓度进行测量，来研究其与最近12个月喘息的关系。哮喘是通过自我诊断确定和对甘露醇的气道高反应性（BHR）证实。
结果：145个工作场所采集到了粉尘样品，92个工作场所 有动物暴露在，53个没有暴露。暴露组由412名受试者组成，非暴露组包括339名受试者。有动物的工作场所相比无动物暴露组中有较高浓度的内毒素，有动物的工作场所内毒素浓度中位数为34.2内毒素单位（EU）每毫克粉尘（间距范围，12.6-65.4），无动物暴露组内毒素浓度中位数10.2EU/毫克粉尘（四分位距，2.6-22.2）（P＜0.001＝。高浓度的内毒素（高于整个样本中位数，20.4EU/毫克）与喘息的患病率增加（P＜0.001＝有关，即暴露于高浓度的内毒素的工人中有61%报告在过去12个月有喘息，暴露于低内毒素浓度的工人只有29%的有喘息。内毒素的浓度与自我报道的哮喘或BHR确认的哮喘无关。
结论：暴露于内毒素与较高的喘息发病率有关，但与由甘露醇支气管激发试验确认的或自我报告确认的哮喘无关。对这些工人采取预防措施是必要的。
（杨冬 审校）
BMC Pulm Med. 2016 May 6;16(1):69. doi: 10.1186/s12890-016-0233-1.
Exposure to high endotoxin concentration increases wheezing prevalence among laboratory animal workers: a cross-sectional study.
Freitas AS1, Simoneti CS2, Ferraz E2, Bagatin E3, Brandão IT4, Silva CL4, Borges MC2, Vianna EO5.
Author information
Abstract
BACKGROUND:Endotoxin from Gram-negative bacteria are found in different concentrations in dust and on the ground of laboratories dealing with small animals and animal houses.
METHODS:Cross-sectional study performed in workplaces of two universities. Dust samples were collected from laboratories and animal facilities housing rats, mice, guinea pigs, rabbits or hamsters and analyzed by the "Limulus amebocyte lysate" (LAL) method. We also sampled workplaces without animals. The concentrations of endotoxin detected in the workplaces were tested for association with wheezing in the last 12 months, asthma defined by self-reported diagnosis and asthma confirmed by bronchial hyperresponsiveness (BHR) to mannitol.
RESULTS:Dust samples were obtained at 145 workplaces, 92 with exposure to animals and 53 with no exposure. Exposed group comprised 412 subjects and non-exposed group comprised 339 subjects. Animal-exposed workplaces had higher concentrations of endotoxin, median of 34.2 endotoxin units (EU) per mg of dust (interquartile range, 12.6-65.4), as compared to the non-exposed group, median of 10.2 EU/mg of dust (interquartile range, 2.6-22.2) (p < 0.001). The high concentration of endotoxin (above whole sample median, 20.4 EU/mg) was associated with increased wheezing prevalence (p < 0.001), i.e., 61 % of workers exposed to high endotoxin concentration reported wheezing in the last 12 months compared to 29 % of workers exposed to low endotoxin concentration. The concentration of endotoxin was not associated with asthma report or with BHR confirmed asthma.
CONCLUSION:Exposure to endotoxin is associated with a higher prevalence of wheezing, but not with asthma as defined by the mannitol bronchial challenge test or by self-reported asthma. Preventive measures are necessary for these workers.
KEYWORDS:Animal handler; Endotoxin; Occupational disease; Wheezing
BMC Pulm Med. 2016 May 6;16(1):69. doi: 10.1186/s12890-016-0233-1.
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