大豆苷和橙皮素对分离的致敏和非致敏豚鼠气管解痉的相互作用
2016/05/31
摘要
中药（TCM）葛根和陈皮常联合用药，用于缓解感冒、发热、支气管炎、咳嗽的治疗。它们含有大豆苷和橙皮素，这两种物质分别为磷酸二酯酶家族（PDE）3（PDE3）和4（PDE4）的选择性抑制剂。在被动致敏的人气道，过敏原诱导的收缩据报道只有PDE3和PDE4同时抑制时才能被抑制，而不是由单一的酶抑制。因此，我们有兴趣研究大豆苷和橙皮素其解痉作用在分离的致敏和非致敏豚鼠气管的相互作用，来弄清楚这两个组织之间的差异，因为中药处方对有无过敏性哮喘的患者的作用往往是不同的。将卵清蛋白（OVA）皮下注射到豚鼠腿做成致敏豚鼠。致敏后，对致敏气管的基线和累积OVA引起的收缩等距记录在多波动描记器上。同样地，组胺（30μM）诱导的非致敏豚鼠气管的强直性收缩也被记录。用isobole法分析大豆苷和橙皮素之间的拮抗作用和协同作用。isoboles显示大豆甘和橙皮素在基线舒张作用和OVA（100μg/ml）诱导的致敏性豚鼠气管收缩作用上相互拮抗。相反地，isobole显示大豆苷和橙皮素对组胺诱导的非致敏豚鼠气管的强直性收缩的舒张作用具有协同效应。这些结果表明，葛根和陈皮的联合用药对缓解感冒、发烧、支气管炎和咳嗽是有效的，但可能对过敏性哮喘患者的影响不大。
（杨冬 审校）
Front Pharmacol. 2016 Mar 29;7:75. doi: 10.3389/fphar.2016.00075. eCollection 2016.
Interaction Between Daidzein and Hesperetin on Antispasmodic Action in Isolated Sensitized and Non-sensitized Guinea-Pig Tracheas.
Shih CH1, Chang TY2, Ko WC2.
Author information
Abstract
In traditional Chinese medicine (TCM), a combination of kudzu and Chen-Pi is frequently prescribed for relieving colds, fever, bronchitis, and cough. It contains daidzein and hesperetin, selective inhibitors of family 3 (PDE3), and 4 (PDE4) of phosphodiesterases (PDEs), respectively. In passively sensitized human airways, allergen-induced contraction was reported to be inhibited only by the simultaneous inhibition of PDE3 and PDE4, but not by single inhibition of either isozyme. Therefore, we are interested in investigating the interaction between daidzein and hesperetin on their antispasmodic effects in the isolated sensitized and non-sensitized guinea-pig tracheas, to clarify the difference between these two tissues, because effects of TCM prescription on patients with or without allergic asthma are often different. Guinea-pigs were sensitized by subcutaneous injection of ovalbumin (OVA) into legs. After sensitization, the baseline and cumulative OVA-induced contractions of the sensitized trachea were isometrically recorded on a polygraph. In the same way, the histamine (30 μM)-induced tonic contraction of non-sensitized guinea-pig trachea was recorded. The isobole method was used to analyze the antagonism and synergism between daidzein and hesperetin. The isoboles showed antagonism between daidzein and hesperetin on baseline relaxant effect and OVA (100 μg/ml)-induced contraction in the sensitized guinea-pig trachea. In contrast, the isobole showed synergism between daidzein and hesperetin on the relaxant effect of histamine-induced tonic contraction in non-sensitized guinea-pig trachea. These results suggest that the combination of kudzu and Chen-Pi for relieving colds, fever, bronchitis and cough is effective in patients without, but might show little effect in patients with allergic asthma.
KEYWORDS:Guinea-pig trachea; antagonism; daidzein; hesperetin; isobole; synergism
Front Pharmacol. 2016 Mar 29;7:75. doi: 10.3389/fphar.2016.00075. eCollection 2016.
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