哮喘联盟

   摘要
   本研究的目的是确定在哮喘模型中有氧运动训练（AT）对糖皮质激素受体（GR）和抗炎细胞因子的表达的作用。 BALB/ c小鼠随机分为对照组（CT;非致敏/未经训练），有氧训练组（AT;非致敏/经训练），卵清蛋白组（OVA;致敏/未经训练），和OVA+ AT组（致敏/经训练）。 OVA组通过吸入接受OVA，而AT组完成1，3或7天的练习（60分钟/次）。 对GR，IL-4，IL-5，IL-10，IL-1受体拮抗剂，NF-κB，TGF-β，VEGF，ICAM-1，VCAM-1的表达;嗜酸性粒细胞计数;气道重塑（AR）特征〔气道平滑肌（ASM）和上皮的厚度以及胶原纤维的沉积〕进行定量。OVA致敏诱发GR的表达下降并增加嗜酸性粒细胞，IL-4，IL-5，NF-κB，TGF-β，VEGF，ICAM-1，VCAM-1的表达和AR的特征（P <0.05）。3天之后，AT逆转OVA诱导的GR表达的降低，并随后诱导IL-10和IL-1受体拮抗剂的表达增加（第七天）。与此相反，嗜酸性粒细胞迁移，NF-κB，IL-4，IL-5，TGF-β，RANTES，VEGF，ICAM-1，VCAM-1的表达，以及AR特征（P <0.05）减少。 AT在哮喘的动物模型中增加GR和抗炎细胞因子（IL-10和IL-1受体拮抗剂）的表达，并减少炎症介质的表达，缓解气道炎症。

 

（苏欣 审校）
Scand J Med Sci Sports. 2015 Feb 4. doi: 10.1111/sms.12411. [Epub ahead of print]


 

Exercise reverses OVA-induced inhibition of glucocorticoid receptor and increases anti-inflammatory cytokines in asthma.
 

Silva RA1, Almeida FM, Olivo CR, Saraiva-Romanholo BM, Martins MA, Carvalho CR.

ABSTRACT
The purpose of this study was to determine the effect of aerobic exercise training (AT) on the expression of glucocorticoid receptors (GR) and anti-inflammatory cytokines in an asthma model. BALB/c mice were divided into groups control (CT; nonsensitized/nontrained), aerobic training (AT; nonsensitized/trained), ovalbumin (OVA; sensitized/not trained), and OVA+AT (sensitized/trained). OVA groups received OVA by inhalation, and the AT groups completed 1, 3, or 7 days of exercise (60 min/session). Expression of GR, IL-4, IL-5, IL-10, IL-1ra, NF-κB, TGF-β, VEGF, ICAM-1, VCAM-1; eosinophils counting; and airway remodeling (AR) features [airway smooth muscle (ASM) and epithelial thickness and collagen fiber deposition] were quantified. OVA sensitization induced a decrease in the expression of GR and increases in the eosinophil, IL-4, IL-5, NF-κB, TGF-β, VEGF, ICAM-1, VCAM-1, and AR features (P < 0.05). After 3 days, AT reversed the OVA-induced reduction in the expression of GR, and subsequently induced increases in the expression of IL-10 and IL-1ra (seventh day). In contrast, the eosinophil migration, the expression of NF-κB, IL-4, IL-5, TGF-β, RANTES, VEGF, ICAM-1, VCAM-1, and the AR features (P < 0.05) were reduced. AT increases the expression of GR and anti-inflammatory cytokines (IL-10 and IL-1ra) and reduces the expression of inflammatory mediators and airway inflammation in an animal model of asthma.

Scand J Med Sci Sports. 2015 Feb 4. doi: 10.1111/sms.12411. [Epub ahead of print]